Results 71 to 80 of about 371,884 (292)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Flexural–torsional behavior of thin-walled composite box beams using shear-deformable beam theory [PDF]

, 2008
This paper presents a flexural–torsional analysis of thin-walled composite box beams. A general analytical model applicable to thin-walled composite box beams subjected to vertical and torsional loads is developed.
Lee, Jaehong, Vo, Thuc
core   +1 more source

Parametric matrix models

Nature Communications
We present a general class of machine learning algorithms called parametric matrix models. In contrast with most existing machine learning models that imitate the biology of neurons, parametric matrix models use matrix equations that emulate physical ...
Patrick Cook, Danny Jammooa, Morten Hjorth-Jensen, Daniel D. Lee, Dean Lee   +4 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Effect of terahertz irradiation on DNA damage repair in living cells

FEBS Open Bio, EarlyView.
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno, Shota Yamazaki, Hiromichi Hoshina, Masahiko Harata   +3 more
wiley   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Abrupt structural transition in exotic molybdenum isotopes unveils an isospin-symmetric island of inversion

Nature Communications
Like electrons in atoms, protons and neutrons in nuclei occupy orbitals in a shell structure with energy gaps at magic numbers. Radioactive-beam experiments revealed the disappearance of magic numbers in some neutron-rich isotopes.
J. Ha, F. Recchia, S. M. Lenzi, H. Iwasaki, D. D. Dao, F. Nowacki, A. Revel, P. Aguilera, G. de Angelis, J. Ash, D. Bazin, M. A. Bentley, S. Biswas, S. Carollo, M. L. Cortes, R. Elder, R. Escudeiro, P. Farris, A. Gade, T. Ginter, M. Grinder, J. Li, D. R. Napoli, S. Noji, J. Pereira, S. Pigliapoco, A. Pompermaier, A. Poves, K. Rezynkina, A. Sanchez, R. Wadsworth, D. Weisshaar   +31 more
doaj   +1 more source

Nonlinear Finite Element Analysis of High Strength Lightweight Concrete Beams [PDF]

Engineering and Technology Journal, 2010
This research work presents a nonlinear finite element investigation on thebehavior of lightweight reinforced concrete beams. This investigation is carriedout in order to get a better understanding of their behavior throughout the entireloading history ...
Farked Kais Ibrahim
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source