Results 51 to 60 of about 110,049 (267)

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Plastic rotation capacity of lightweight-aggregate concrete beams

Journal of Civil Engineering and Management, 2016
This article describes a study on the plastic behaviour of lightweight-aggregate concrete beams. The experimental results of nineteen simply supported beams previously tested by the authors were used in this study.
Luís F. A. Bernardo, Miguel C. S. Nepomuceno, Hugo A. S. Pinto   +2 more
doaj   +1 more source

Layer-dependent magnetic phase diagram in Fe n GeTe2 (3 ≤ n ≤ 7) ultrathin films

Communications Physics, 2022
2D magnetism offers the prospect of ordered and controllable magnetic states at reduced dimensions and there is a desire to obtain materials which can realise these properties at room temperature.
Qinxi Liu, Jianpei Xing, Zhou Jiang, Yu Guo, Xue Jiang, Yan Qi, Jijun Zhao   +6 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Enhanced production of 60Fe in massive stars

Nature Communications
Massive stars are a major source of chemical elements in the cosmos, ejecting freshly produced nuclei through winds and core-collapse supernova explosions into the interstellar medium.
A. Spyrou, D. Richman, A. Couture, C. E. Fields, S. N. Liddick, K. Childers, B. P. Crider, P. A. DeYoung, A. C. Dombos, P. Gastis, M. Guttormsen, K. Hermansen, A. C. Larsen, R. Lewis, S. Lyons, J. E. Midtbø, S. Mosby, D. Muecher, F. Naqvi, A. Palmisano-Kyle, G. Perdikakis, C. Prokop, H. Schatz, M. K. Smith, C. Sumithrarachchi, A. Sweet   +25 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Parametric matrix models

Nature Communications
We present a general class of machine learning algorithms called parametric matrix models. In contrast with most existing machine learning models that imitate the biology of neurons, parametric matrix models use matrix equations that emulate physical ...
Patrick Cook, Danny Jammooa, Morten Hjorth-Jensen, Daniel D. Lee, Dean Lee   +4 more
doaj   +1 more source