Results 171 to 180 of about 56,257 (250)

Transient Transfection of Rolling‐Circle Amplified DNA in Biomanufacturing‐Relevant Mammalian Cell Lines: A Comparison of Transfection Conditions for Optimal Protein Expression

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Rolling‐circle amplification (RCA) is a completely synthetic and rapid mechanism for scaling‐up bulk DNA that eliminates requirements for large‐scale bacterial fermentation. Several reports have described the surprising ability of large unprocessed (hyperbranched) rolling circle‐amplified DNA (RCA DNA) to effectively transfect cultured cells ...
Evelina Loghin, Erik Kvam, Lisa Lowery, Nathan Hudson, Jenny Keen, Brian Davis, Roger B. Dodd, Christine Surrette, John Nelson   +8 more
wiley   +1 more source

Oral Deuterated Docosahexaenoic Acid Protects Against Onset and Progression of RPE Degeneration in a Mouse Model of Chronic Oxidative Stress. [PDF]

Invest Ophthalmol Vis Sci
Foshe S, Anderson BD, Song Y, Shi S, Lee S, Bell BA, Park HG, Brenna JT, Shchepinov M, Dunaief JL.   +9 more
europepmc   +1 more source

Enzymatic synthesis of acylglycerides from agroindustrial residues: process design and simplified techno‐economic assessment

Biofuels, Bioproducts and Biorefining, EarlyView.
Abstract This study developed an enzymatic process to add value to palm oil fatty acid distillate (PFAD) and glycerol, resulting in the production of monoacylglycerides (MAG) and diacylglycerides (DAG). The biocatalyst loading (Novozym 435) and molar ratio of reagents were evaluated to obtain a suitable reaction condition in terms of space–time yield ...
Ronaldo Rodrigues de Sousa, Isabelle de Souza Barbosa, Ana Cristina de Araújo Collaço, Denise Maria Guimarães Freire, Viridiana Santana Ferreira‐Leitão   +4 more
wiley   +1 more source

Pre-existing particle composition controls the initial viscosity and aging of α-pinene-derived secondary organic aerosol.

Environ Sci Atmos
Kolozsvari KRA, Garner NM, Bell DM, Top J, Ammann M, Ault AP.   +5 more
europepmc   +1 more source

Ether lipids influence cancer cell fate by modulating iron uptake. [PDF]

Nat Commun
Mansell RP, Müller S, Yang JS, Innes-Gold S, Das S, Reinhardt F, Sigmund KJ, Phadnis VV, Wan Z, Stark J, Hwang D, Sindikubwabo F, Syx L, Servant N, Eaton EN, Sampaio JL, Bell GW, Thiru P, Viravalli A, Deik A, Clish CB, Hammond PT, Kamm RD, Cohen AE, Levental I, Boehnke N, Hsu VW, Levental KR, Rodriguez R, Weinberg RA, Henry WS.   +30 more
europepmc   +1 more source

Physiologically‐based pharmacokinetic modelling of uridine 5′‐diphosphoglucorosultransferase (UGT) substrate drugs in pregnant women

British Journal of Clinical Pharmacology, EarlyView.
Aims While pregnancy‐related changes in phase I enzyme activity are well‐documented, less is known about the impact on phase II enzymes. This study aimed to test the hypothesis that changes in the pharmacokinetics (PK) of uridine 5′‐diphosphoglucuronosyltransferase (UGT) substrates during pregnancy result from altered enzyme expression or activity ...
William Saffaf, Ymke van der Velde, Paul R. V. Malik, Jelmer R. Prins, Sanne J. Gordijn, Daan J. Touw, Paola Mian   +6 more
wiley   +1 more source

Discovery of a bell-shaped dose response curve to melanin-concentrating hormone in the 3T3-L1 adipocyte model: low-dose MCH facilitates adipogenesis. [PDF]

Adipocyte
Cook LB, King CM, Abdullah HY.
europepmc   +1 more source

Activism in the arts: Co‐researching cultural inequalities with young people during the COVID‐19 pandemic

British Educational Research Journal, EarlyView.
Abstract This paper explores the growing influence of young people's activism in UK museums and its educational implications. It draws on a five‐year collaborative programme (2019–2023) with young people of colour (16–28) in a university museum setting, focusing on a Young Collective established to address cultural inequalities.
Sadia Habib
wiley   +1 more source

Measurement of ion acceleration and diffusion in a laser-driven magnetized plasma. [PDF]

Nat Commun
Chu JTY, Halliday JWD, Heaton C, Moczulski K, Blazevic A, Schumacher D, Metternich M, Nazary H, Arrowsmith CD, Bell AR, Beyer KA, Bott AFA, Campbell T, Hansen E, Lamb DQ, Miniati F, Neumayer P, Palmer CAJ, Reville B, Reyes A, Sarkar S, Scopatz A, Spindloe C, Stuart CB, Wen H, Tzeferacos P, Bingham R, Gregori G.   +27 more
europepmc   +1 more source

Mediated Electrochemical Probing and Machine Learning for Cysteine and Reduced Monoclonal Antibody Quantification

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Biopharmaceutical manufacturing requires robust analytics and process controls throughout production to insure high yield of quality products. New methodologies for rapidly accessing and integrating data‐rich information from complex dynamic biological environments are of great interest.
Kayla Chun, Varun Kochar, Evelyn Jaskowiak, Chen‐Yu Chen, Divya Muthusamy, Dana Motabar, Eunkyoung Kim, Gregory F. Payne, William E. Bentley   +8 more
wiley   +1 more source