Results 231 to 240 of about 6,669,365 (283)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Structural basis for prostaglandin and drug transport via SLCO2A1. [PDF]

Nat Commun
Joshi C, Deme JC, Nakamura Y, Hsu WT, Goult JD, Kato T, Parker JL, Biggin PC, Lea SM, Nakanishi T, Newstead S.   +10 more
europepmc   +1 more source

Domain associated with zinc fingers‐containing NF90‐NF45 complex inhibits m6A modification of primary microRNA by suppressing METTL3/14 activity

FEBS Open Bio, EarlyView.
NF90–NF45 functions as a negative regulator of methyltransferase‐like 3/14 (METTL3/14)‐mediated N6‐methyladenosine (m6A) modification on primary microRNAs (pri‐miRNAs). NF90–NF45 binds to anti‐oncogenic pri‐miRNAs and inhibits their m6A modification, thereby suppressing the biogenesis of anti‐oncogenic miRNAs.
Takuma Higuchi, Shunsuke Morioka, Keiko Morisawa, Kazutsugu Matsukawa, Shingo Ashida, Takeshi Suzuki, Shuji Sakamoto   +6 more
wiley   +1 more source

Pioglitazone plus (−)‐epigallocatechin gallate: a novel approach to enhance osteogenic performance in aged bone marrow mesenchymal stem cells

FEBS Open Bio, EarlyView.
Aged human bmMSCs are seeded in the scaffold. Osteoblastic induction can slightly increase cell's bone‐forming activity to produce bone‐like tissues, shown as the sporadic xylenol orange‐stained spots (the lower left image). Notably, pioglitazone plus EGCG co‐treatment dramatically increases cell's bone‐forming activity and bone‐like tissue production (
Ching‐Yun Chen, Jia‐Ci Jhang, Chun‐Yi Peng, Quốc Cường Nguyễn, Feng‐Huei Lin, Hung‐Ming Wu, Shankung Lin   +6 more
wiley   +1 more source

A virtual screening and molecular dynamics approach in search of novel antibiotic chemotypes. [PDF]

PLoS One
Noor T, Schultz DC, Zhai Y, Snoke HE, Noh S, Seabra G, Lee RE, Jeong KC, Li C, Siddiqi AR.   +9 more
europepmc   +1 more source

Mechanism of Hsp70 activation: How J-domain proteins push for ATP hydrolysis. [PDF]

PLoS Comput Biol
Olewniczak M, Pitek M, Czub J, Marszalek J, Nierzwicki Ł, Tomiczek B.   +5 more
europepmc   +1 more source

Structure-Guided Optimization and Biological Validation of 1,3,4-Thiadiazole-Based SIRT2 Inhibitors Reinforcing Channel Entrance Interactions. [PDF]

Drug Dev Res
Aksel AB, Ozgencil F, Bakar-Ates F, Gunindi HB, Massarotti A, Ozkan E, Kaya SG, Gozelle M, Ozkan Y, Eren G.   +9 more
europepmc   +1 more source

Biophysical characterization of <i>sp</i>Cas9 binding and cleavage using real-time electronic biosensors.

Sens Diagn
Ban DK, Parate K, Bharadwaj D, Wong A, Schoch L, Visk K, Aran K.   +6 more
europepmc   +1 more source