Results 141 to 150 of about 1,191,896 (339)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Biomimetic and Compressible Wood Phase Change Gel With Hierarchically Aligned Lamellar Structure for Controlled Thermal Management

open access: yesCarbon Energy
The recovery and utilization of ubiquitous low‐grade heat are crucial for mitigating the fossil energy crisis. However, uncontrolled spontaneous heat dissipation limits its practical application.
Jiazuo Zhou   +13 more
doaj   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

open access: yesFEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito   +3 more
wiley   +1 more source

Hierarchical VOx@Wood Aerogel Electrodes with Tunable Valence States for Enhanced Energy Storage

open access: yesNanomaterials
Vanadium-based electrode materials are limited in practical applications, due to their low energy density, cycling instability, and poor electrochemical stability.
Yu Wang   +7 more
doaj   +1 more source

A bio-inspired memory device based on interfacing Physarum polycephalum with an organic semiconductor [PDF]

open access: gold, 2014
Agostino Romeo   +5 more
openalex   +1 more source

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

open access: yesFEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source

End-of-Life Options for Bio-Based Plastics in a Circular Economy—Status Quo and Potential from a Life Cycle Assessment Perspective [PDF]

open access: gold, 2020
Sebastian Spierling   +5 more
openalex   +1 more source

Citizen Science for Bio-indication: Development of a Community-Based Index of Ecosystem Integrity for Assessing the Status of Afrotropical Riverine Ecosystems [PDF]

open access: gold, 2021
Christopher Mulanda Aura   +7 more
openalex   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

open access: yesFEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso   +4 more
wiley   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

open access: yesFEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima   +4 more
wiley   +1 more source

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