Results 201 to 210 of about 193,280 (312)

Synthesis of <i>Arapaima gigas</i> Growth Hormone (ag-GH) in HEK 293 Cells: Its Purification and Characterization via In Vivo Bioassay in Dwarf "Little" Mice. [PDF]

open access: yesMolecules
Lima ER   +11 more
europepmc   +1 more source

Temperature interacts with jack pine host defences to influence the growth of mountain pine beetle‐associated fungi

open access: yesAgricultural and Forest Entomology, EarlyView.
Optimal growing temperatures varied with mountain pine beetle‐associated fungal species. There was only a marginal impact of temperature on the response of fungi to defensive monoterpenes of novel host pine. Interspecific facilitation occurred for Leptiographuim longiclavatum when grown in Ophiostoma montium‐induced treatments at optimal temperatures ...
Adrienne C. Bailey   +4 more
wiley   +1 more source

Assessing the host plants of the invasive apricot aphid, Myzus mumecola (Hemiptera: Aphididae)

open access: yesAgricultural and Forest Entomology, EarlyView.
We investigated the host plants of the invasive apricot aphid Myzus mumecola (Hemiptera: Aphididae), an emerging pest species in Europe, by combining molecular and ecological approaches. The winter host plant was confirmed as apricot by both methods.
Marta Chignola   +2 more
wiley   +1 more source

Measuring Propidium Iodide Brightness as a Function of Viability: A High‐Throughput Quantitative Assessment of Hepatic Spheroids

open access: yesArtificial Organs, EarlyView.
The graphical abstract illustrates our rapid PI‐based spheroid viability assay: hepatocyte spheroids are formed and stained with AO/PI, imaged after a 15‐min incubation, and analyzed for PI brightness normalized to area. This metric correlates strongly with functional urea/DNA output, validating PI intensity as a fast, reliable indicator of spheroid ...
Michael G. Megaly   +11 more
wiley   +1 more source

Integrating artificial intelligence and manual curation to enhance bioassay annotations in ChEMBL. [PDF]

open access: yesJ Cheminform
Smit I   +8 more
europepmc   +1 more source

Rapid generation of prion disease models using AAV‐delivered PrP variants in knockout mice

open access: yesBrain Pathology, EarlyView.
We developed a rapid AAV‐based system to generate prion disease models in weeks rather than months. Following systemic AAV9P31 delivery of modified PrP to knockout mice, we achieved brain‐wide expression and successful propagation of both classical (RML) and atypical (GSS‐A117V) prion strains.
Maitena San‐Juan‐Ansoleaga   +11 more
wiley   +1 more source

The L108I polymorphism in mouse prion protein drives spontaneous disease and enhances transmission of atypical and classical prion strains

open access: yesBrain Pathology, EarlyView.
A single amino acid change (L108I) combined with PrP overexpression drives spontaneous atypical prion formation in mice, enabling also efficient propagation of diverse prion strains. This model allows studying how spontaneous prion diseases arise and provides powerful tools for investigating strain emergence, transmission barriers, and mechanisms ...
Hasier Eraña   +20 more
wiley   +1 more source

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