Results 61 to 70 of about 61,729 (257)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Determination of Baseline Data of Physicochemical and Microbiological Quality of Surface Waters in Nsit Ibom Local Government Area of Akwa Ibom State, Nigeria

Journal of Applied Sciences and Environmental Management
Surface waters in Nsit Ibom Local Government Area of Akwa Ibom State are the predominant sources of potable water for the rapidly increasing population in the area with the presence of three tertiary institutions.
B. P. Akpan, A. L. Udo, E. S. Akpan
doaj  

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Modelling biochemical oxygen demand using improved neuro-fuzzy approach by marine predators algorithm. [PDF]

Environ Sci Pollut Res Int, 2023
Adnan RM, Dai HL, Kisi O, Heddam S, Kim S, Kulls C, Zounemat-Kermani M.   +6 more
europepmc   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Pengaruh Bambu Untuk Pipa Distribusi Air Minum Terhadap Kualitas Air (Studi Kasus Distribusi Air Minum Di Paweden, Karangkobar, Banjarnegara)

Dinamika Rekayasa, 2005
This research was aimed to know bamboo influence to the physical, and chemical characteristic, and water quality. Parameters observed are temperature, color, taste, odor, pH,DO (dissolved oxygen), BOD (biochemical oxygen demand) and COD (biochemical ...
Nastain, Gito Sugiyanto, Purwanto Bekti Santoso   +2 more
doaj  

Predicting biochemical oxygen demand in wastewater treatment plant using advance extreme learning machine optimized by Bat algorithm. [PDF]

Heliyon, 2023
Mekaoussi H, Heddam S, Bouslimanni N, Kim S, Zounemat-Kermani M.   +4 more
europepmc   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

A SOFTWARE SENSOR FOR IN-SITU MONITORING OF THE 5-DAY BIOCHEMICAL OXYGEN DEMAND

Rudarsko-geološko-naftni Zbornik, 2018
Due to the time-consuming procedure for determining the 5-day biochemical oxygen demand (BOD5), the present study developed two software sensors based on artificial intelligence techniques to estimate this indicator instantaneously.
Rana Kasem, Dimah ALabdeh, Roohollah Noori, Abdulreza Karbassi   +3 more
doaj   +1 more source