Results 11 to 20 of about 39 (36)

A New Platelet‐Aggregation‐Inhibiting Factor Isolated from Bothrops moojeni Snake Venom

BioMed Research International, Volume 2017, Issue 1, 2017., 2017
This work reports the purification and functional characterization of BmooPAi, a platelet‐aggregation‐inhibiting factor from Bothrops moojeni snake venom. The toxin was purified by a combination of three chromatographic steps (ion‐exchange on DEAE‐Sephacel, molecular exclusion on Sephadex G‐75, and affinity chromatography on HiTrap™ Heparin HP ...
Bruna Barbosa de Sousa, Carla Cristine Neves Mamede, Mariana Santos Matias, Déborah Fernanda da Cunha Pereira, Mayara Ribeiro de Queiroz, Edigar Henrique Vaz Dias, Anielle Christine Almeida Silva, Noelio Oliveira Dantas, Júnia de Oliveira Costa, Fábio de Oliveira, Ji-Fu Wei   +10 more
wiley   +1 more source

Alteration of ROS Homeostasis and Decreased Lifespan in S. cerevisiae Elicited by Deletion of the Mitochondrial Translocator FLX1

BioMed Research International, Volume 2014, Issue 1, 2014., 2014
This paper deals with the control exerted by the mitochondrial translocator FLX1, which catalyzes the movement of the redox cofactor FAD across the mitochondrial membrane, on the efficiency of ATP production, ROS homeostasis, and lifespan of S. cerevisiae.
Teresa Anna Giancaspero, Emilia Dipalo, Angelica Miccolis, Eckhard Boles, Michele Caselle, Maria Barile, Dina Bellizzi   +6 more
wiley   +1 more source

Rapid Purification of a New P‐I Class Metalloproteinase from Bothrops moojeni Venom with Antiplatelet Activity

BioMed Research International, Volume 2014, Issue 1, 2014., 2014
The present study aimed to evaluate the proteolytic and biological activities of a new metalloproteinase from B. moojeni venom. The purification of BmooMPα‐II was carried out through two chromatographic steps (ion‐exchange and affinity). BmooMPα‐II is a monomeric protein with an apparent molecular mass of 22.5 kDa on SDS‐PAGE 14% under nonreducing ...
Mayara R. de Queiroz, Carla C. Neves Mamede, Kelly C. Fonseca, Nadia C. G. de Morais, Bruna B. de Sousa, Norival A. Santos-Filho, Marcelo E. Beletti, Eliane C. Arantes, Leonilda Stanziola, Fábio de Oliveira, Jozef Anné   +10 more
wiley   +1 more source

Diseño y preparación de formas farmacéuticas sólidas de benznidazol para el tratamiento de la enfermedad de Chagas [PDF]

Revista Argentina de Salud Pública, 2013
n Argentina se emplea el benznidazol como terapéutica de primera línea para el tratamiento etiológico del Chagas. Desde su lanzamiento (hace más de 40 años), sólo se dispone de comprimidos convencionales de 100 mg; no se han desarrollado nuevas formas ...
Sonia, Tarragona , Claudio, Salomón, Álvaro, Jiménez-Kairuz , María, Lamas, Silvina , Orlandi, Darío, Leonardi , Norma, Maggia, Alejandro ,Paredes, Carolina, Romañuk , Mónica, García   +9 more
doaj  

Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation

BioMed Research International, Volume 2014, Issue 1, 2014., 2014
In this paper, we describe the purification/characterization of BmooAi, a new toxin from Bothrops moojeni that inhibits platelet aggregation. The purification of BmooAi was carried out through three chromatographic steps (ion‐exchange on a DEAE‐Sephacel column, molecular exclusion on a Sephadex G‐75 column, and reverse‐phase HPLC chromatography on a C2/
Mayara Ribeiro de Queiroz, Carla Cristine N. Mamede, Nadia Cristina G. de Morais, Kelly Cortes Fonseca, Bruna Barbosa de Sousa, Thaís M. Migliorini, Déborah Fernanda C. Pereira, Leonilda Stanziola, Leonardo A. Calderon, Rodrigo Simões-Silva, Andreimar Martins Soares, Fábio de Oliveira, Phillip I. Bird   +12 more
wiley   +1 more source

Strychnos pseudoquina and Its Purified Compounds Present an Effective In Vitro Antileishmanial Activity

Evidence-Based Complementary and Alternative Medicine, Volume 2013, Issue 1, 2013., 2013
The development of new and cost‐effective alternative therapeutic strategies to treat leishmaniasis has become a high priority. In the present study, the antileishmanial activity of Strychnos pseudoquina St. Hil. was investigated and pure compounds that presented this biological effect were isolated. An ethyl acetate extract was prepared, and it proved
Paula Sousa Lage, Pedro Henrique Rocha de Andrade, Amanda de Santana Lopes, Miguel Angel Chávez Fumagalli, Diogo Garcia Valadares, Mariana Costa Duarte, Daniela Pagliara Lage, Lourena Emanuele Costa, Vivian Tamietti Martins, Tatiana Gomes Ribeiro, José Dias de Souza Filho, Carlos Alberto Pereira Tavares, Rodrigo Maia de Pádua, João Paulo Viana Leite, Eduardo Antonio Ferraz Coelho, Virginia S. Martino   +15 more
wiley   +1 more source

ASPic‐GeneID: A Lightweight Pipeline for Gene Prediction and Alternative Isoforms Detection

BioMed Research International, Volume 2013, Issue 1, 2013., 2013
New genomes are being sequenced at an increasingly rapid rate, far outpacing the rate at which manual gene annotation can be performed. Automated genome annotation is thus necessitated by this growth in genome projects; however, full‐fledged annotation systems are usually home‐grown and customized to a particular genome.
Tyler Alioto, Ernesto Picardi, Roderic Guigó, Graziano Pesole, Tao Huang   +4 more
wiley   +1 more source

A Regulatory Role of NAD Redox Status on Flavin Cofactor Homeostasis in S. cerevisiae Mitochondria

Oxidative Medicine and Cellular Longevity, Volume 2013, Issue 1, 2013., 2013
Flavin adenine dinucleotide (FAD) and nicotinamide adenine dinucleotide (NAD) are two redox cofactors of pivotal importance for mitochondrial functionality and cellular redox balance. Despite their relevance, the mechanism by which intramitochondrial NAD(H) and FAD levels are maintained remains quite unclear in Saccharomyces cerevisiae. We investigated
Teresa Anna Giancaspero, Vittoria Locato, Maria Barile, Cristina Mazzoni   +3 more
wiley   +1 more source

Bmoo FIBMP‐I: A New Fibrinogenolytic Metalloproteinase from Bothrops moojeni Snake Venom

International Scholarly Research Notices, Volume 2012, Issue 1, 2012., 2012
A new fibrinogenolytic metalloproteinase (Bmoo FIBMP‐I) was purified from Bothrops moojeni snake venom. This enzyme was isolated through a combination of three chromatographic steps (ion‐exchange, molecular exclusion, and affinity chromatography). Analyses by reverse phase chromatography, followed by mass spectrometry, showed the presence of enzyme ...
F. S. Torres, B. Rates, M. T. R. Gomes, C. E. Salas, A. M. C. Pimenta, F. Oliveira, M. M. Santoro, M. E. de Lima, A. Cruz, F. Ducancel, Y. N. Utkin   +10 more
wiley   +1 more source

TNF/TNFR1 is a Key Regulator of Prolonged Fasting‐Induced Decrease in Adipose Tissue

The FASEB Journal, Volume 40, Issue 1, 15 January 2026.
Fasting triggers an inflammatory response and increases sympathetic tone in white adipose tissue (WAT), promoting fat breakdown and tissue remodeling. Fasting sparks an inflammatory response that underpins the reduction in adipocyte size and WAT remodeling.
Amanda Carla Clemente de Oliveira, Adma Maciel Babêtto, Mariele Lino Silva, Larisse de Souza Barbosa Lacerda, Thiago de Souza Rodrigues, Marina Chaves de Oliveira, Christine Rouault, Emmanuel L. Gautier, André Gustavo Oliveira, Karine Clément, Mauro Martins Teixeira, Geneviève Marcelin, Adaliene Versiani Matos Ferreira   +12 more
wiley   +1 more source