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Efficient Degradation of Monoacylglycerols by an Engineered Aspergillus oryzae Lipase: Synergistic Effects of sfGFP Fusion and Rational Design [PDF]

Molecules
Monoacylglycerols (MAGs) are significant intermediate byproducts in the hydrolysis of oils and fats. The accumulation of MAGs not only reduces the quality and purity of the final products in biodiesel production and edible oil refining but also poses ...
Yuqing Wang, Fang Liu, Yuxi Tian, Jiazhen Sun, Dawei Liu, Fei Li, Yaping Wang, Ben Rao   +7 more
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Study on the High-Efficiency Expression of Horseradish Peroxidase in Pichia pastoris [PDF]

Molecules
Horseradish peroxidase (HRP) is a heme-containing oxidoreductase with extensive applications in biotechnology, medical diagnostics, and environmental protection. In this study, Pichia pastoris was utilized to produce HRP. Successfully, expression strains
Yaping Wang, Yidan Jing, Weizhen Li, Yuqing Wang, Fei Li, Yimin Qiu, Ben Rao   +6 more
doaj   +2 more sources

Comparative Control of Phyllotreta striolata: Growth-Inhibiting Effects of Chemical Insecticides Versus the Green Advantages of a Biopesticide [PDF]

Insects
Phyllotreta striolata is a major pest of Brassica chinensis, with chemical pesticides being the primary method for pest control. However, their negative impact on crop growth and environmental risks have prompted the search for low-toxicity ...
Fuyong Lin, Musa Hassan Muhammad, Yufei Mao, Fan Zhao, Zixuan Wang, Yongcong Hong, Pumo Cai, Xiong Guan, Tianpei Huang   +8 more
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Nano-biopesticide formulation comprising of silver nanoparticles anchored to Ocimum sanctum: a sustainable approach to pest control in jute farming [PDF]

Scientific Reports
The jute hairy caterpillar, Spilosoma obliqua (Lepidoptera: Erebidae) is considered as one of the major threats to jute cultivation. The best eco-friendly methods to combat these jute pests involve administration of nano-biopesticides, as a successful ...
Avirup Ghosh, Dipanwita Majumdar, Himani Biswas, Anupam Chowdhury, Sanjoy Podder   +4 more
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Acaricidal Activity and Field Efficacy Analysis of the Potential Biocontrol Agent Bacillus vallismortis NBIF-001 against Spider Mites

Microorganisms, 2022
In recent years, spider mites have caused considerable economic losses to global agriculture. However, currently available management strategies are limited because of the rapid development of resistance. In this study, Bacillus vallismortis NBIF-001 was
Lei Zhu, Ling Chen, Yong Min, Fang Liu, Xianqing Liao, Ben Rao, Yimin Qiu, Wei Chen, Kaimei Wang, Ziwen Yang, Ronghua Zhou, Yan Gong, Xiaoyan Liu   +12 more
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Insect α-Amylases and Their Application in Pest Management

Molecules, 2023
Amylase is an indispensable hydrolase in insect growth and development. Its varied enzymatic parameters cause insects to have strong stress resistance. Amylase gene replication is a very common phenomenon in insects, and different copies of amylase genes
Beibei Wang, Daye Huang, Chunxia Cao, Yan Gong   +3 more
doaj   +1 more source

Expression and Surface Display of an Acidic Cold-Active Chitosanase in Pichia pastoris Using Multi-Copy Expression and High-Density Cultivation

Molecules, 2022
Chitosanase hydrolyzes β-(1,4)-linked glycosidic bonds are used in chitosan chains to release oligosaccharide mixtures. Here, we cloned and expressed a cold-adapted chitosanase (CDA, Genbank: MW094131) using multi-copy expression plasmids (CDA1/2/3/4) in
Yanhong Peng, Yaping Wang, Xiaoyan Liu, Ronghua Zhou, Xianqing Liao, Yong Min, Lixin Ma, Ying Wang, Ben Rao   +8 more
doaj   +1 more source

Improving the Activity of Tryptophan Synthetase via a Nucleic Acid Scaffold

Molecules, 2023
Tryptophan synthetase (TSase), which functions as a tetramer, is a typical enzyme with a substrate channel effect, and shows excellent performance in the production of non-standard amino acids, histamine, and other biological derivatives.
Yaping Wang, Xiangyi Wang, Shuhui Niu, Wei Cheng, Xiaoyan Liu, Yong Min, Yimin Qiu, Lixin Ma, Ben Rao, Lei Zhu   +9 more
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Secondary Metabolites from Aspergillus sparsus NBERC_28952 and Their Herbicidal Activities

Plants, 2023
Fungi have been used in the production of a wide range of biologically active metabolites, including potent herbicides. In the search for pesticides of natural origin, Aspergillus sparsus NBERC_28952, a fungal strain with herbicidal activity, was ...
Zhaoyuan Wu, Fang Liu, Shaoyong Ke, Zhigang Zhang, Hongtao Hu, Wei Fang, Shaoyujia Xiao, Yani Zhang, Yueying Wang, Kaimei Wang   +9 more
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Agroactive volatile organic compounds from microbes: Chemical diversities and potentials of application in crop protection

Advanced Agrochem, 2023
Synthetic chemicals have been widely used in modern agriculture as fertilizer, plant growth regulators and pesticides. Although the chemical inputs improved the crop production, it is well known about their negative effects, such as environmental ...
Kaimei Wang, Shaoyong Ke, Wei Fang, Fang Liu, Zhigang Zhang   +4 more
doaj   +1 more source