Results 31 to 40 of about 53,261 (291)

A fast method for analyzing essential protein mutants in human cells

BioTechniques, 2017
Here we developed a complementation method for the study of essential genes in live human cells using the CRISPR/Cas9 system. Proteins encoded by essential genes were expressed using a derivative of the pCEP4 compensating plasmid in combination with Cas9
Frank Dietsch, Mariel Donzeau, Agnes M. Cordonnier, Etienne Weiss, Bruno Chatton, Marc Vigneron   +5 more
doaj   +1 more source

Strategies for Consulting with the Community : The Cases of Four Large Scale Genetic Databases [PDF]

, 2004
[À l'origine dans / Was originally part of : CRDP - Droit, biotechnologie et rapport au ...
Godard, Béatrice, Knoppers, Bartha Maria, Laberge, Claude, Marshall, Jennifer   +3 more
core   +3 more sources

Engineering of Streptomyces lividans for heterologous expression of secondary metabolite gene clusters

Microbial Cell Factories, 2020
Background Heterologous expression of secondary metabolite gene clusters is used to achieve increased production of desired compounds, activate cryptic gene clusters, manipulate clusters from genetically unamenable strains, obtain natural products from ...
Yousra Ahmed, Yuriy Rebets, Marta Rodríguez Estévez, Josef Zapp, Maksym Myronovskyi, Andriy Luzhetskyy   +5 more
doaj   +1 more source

Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts [PDF]

, 2016
Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent ...
Cowan, Don A., Guerrero, Leandro Demián, Le, Phuong Thi, Makhalanyane, Thulani P., Van De Peer, Yves, Vikram, Surendra   +5 more
core   +3 more sources

Warm stratification improves embryos development and seed germination of Cycas revoluta

Advances in Horticultural Science, 2021
The broad objective of this research is to study the effect of warm stratification on Cycas revoluta zygotic embryos length, seed germination and plant development. Four warm treatments were applied to seeds. Our results showed that seeds storage at room
Jalila Benjelloun, Sarah Bouzroud, Zine El Abidine Triqui, Qamar Lahlimi Alami, Rajaa Layachi, Abdelaziz Smouni, Abdelkarim Guedira   +6 more
doaj   +1 more source

Biotechnologie-Gründungen im Ruhrgebiet: Eine vergleichende Analyse [PDF]

, 2005
Auch im Ruhrgebiet sind Anzeichen einer regionalen Häufung von Biotechnologie-Gründern zu beobachten. In qualitativer Hinsicht ist das Ruhrgebiet wenig attraktiv für Gründer aus anderen Regionen und solchen mit hohem Innovationspotenzial.
Engel, Dirk, Heneric, Oliver
core  

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity

Molecular Oncology, EarlyView.
Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung, David Bickel, Lena C. M. Krause, Daria Ezeriņa, Christian Ickes, Julian Wojtachnia, Christine S. Gibhardt, Magdalena Shumanska, Khadija Wahni, Andrea Paluschkiwitz, Julia Malo Pueyo, Ekaterina Baranova, Wim Vranken, Hedwig Stanisz, Ioana Stejerean‐Todoran, Michael P. Schön, Joris Messens, Ivan Bogeski   +17 more
wiley   +1 more source

Over-expression of a chimeric gene of the transcriptional co-activator MBF1 fused to the EAR repressor motif causes developmental alteration in Arabidopsis and tomato [PDF]

, 2008
Transcriptional co-activators of the Multiprotein Bridging Factor1 (MBF1) type belong to a multigenic family that encode key components of the machinery controlling gene expression by communicating between transcription factors and the basal ...
Bouzayen, Mondher, Hommel, Marie, Jaimes-Miranda, Fabiola, Khalil-Ahmad, Qaiser, Latché, Alain, Mila, Isabelle, Pech, Jean-Claude, Pouzet, Cécile, Regad, Farid   +8 more
core   +1 more source

Autosomal Dominant Erythrocytosis Caused by Non‐Renal Erythropoietin (EPO) Due to EPO c.‐136 G>A Germline Mutation

American Journal of Hematology, EarlyView.
A novel erythropoietin (EPO) promoter mutation (c.‐136 G>A) causes autosomal dominant erythrocytosis via non‐renal expression of EPO. ABSTRACT We previously reported a five‐generation kindred with autosomal dominant erythrocytosis associated with a novel germline promoter variant in the erythropoietin (EPO) gene (EPO c.‐136 G>A).
Lucie Lanikova, Dusan Hrckulak, Veronika Zimolova, Felipe R. Lorenzo, Jihyun Song, Katerina Vecerkova, Olga Babosova, Linda Berkova, Vladimir Korinek, Steve Elliott, Josef T. Prchal   +10 more
wiley   +1 more source

Redox‐aktivierte Sonden für Hochkontrastige Live‐Bildgebung Postsynaptischer Neuronaler Gerüste

Angewandte Chemie, EarlyView.
Sylives sind modifizierte fluoreszierende Peptide für eine kontrastreiche Live‐Bildgebung inhibitorischer (Gephyrin) und exzitatorischer (PSD‐95) postsynaptischer Gerüststrukturen in nativen Neuronen. Eine saubere Markierung erfordert niedrige Sondenkonzentrationen im Nanomolarbereich, während die Aufnahme mikromolare Konzentrationen zellpenetrierender
Christiane Huhn, Clémence Mille, Sheng‐Yang Ho, Felix Lützenkirchen, Vladimir Khayenko, Melanie Hein, Christian Werner, Matthias Kneussel, Johannes W. Hell, Christian G. Specht, Hans M. Maric   +10 more
wiley   +1 more source