Results 151 to 160 of about 1,708,201 (311)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

CONCEPTS IN BIOTECHNOLOGY: HISTORY, SCIENCE AND BUSINESS

, 2010
Preface -- Abbreviations and glossary -- Pt. 1. History -- 1. Introduction -- 2. The early period to 1850 -- 3. The period from 1850 to 1890 -- 4. The period from 1890 to 1950 -- 5.
Buchholz, Klaus and Collins, John
core  

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Retraction Note: Biotechnology for propagation and secondary metabolite production in Bacopa monnieri. [PDF]

Appl Microbiol Biotechnol
Sanyal R, Nandi S, Pandey S, Chatterjee U, Mishra T, Datta S, Prasanth DA, Anand U, Mane AB, Kant N, Jha NK, Jha SK, Shekhawat MS, Pandey DK, Dey A.   +14 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Evolving conservation: The role of unconventional approaches to restore contemporary vertebrate populations and genomic biodiversity. [PDF]

J Hered
vonHoldt BM, James M, Gaughran SJ, DeCandia AL, Koepfli KP, Flores D, Songsasen N, Brzeski KE, Jensen EL.   +8 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Technology integration in intensive care medicine through clinical engineering and convergent biotechnology. [PDF]

J Crit Care Med (Targu Mures)
Azamfirei L.
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Retraction Note: Successive use of microorganisms to remove chromium from wastewater. [PDF]

Appl Microbiol Biotechnol
Elahi A, Arooj I, Bukhari DA, Rehman A.
europepmc   +1 more source