Results 121 to 130 of about 164,929 (275)

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Simultaneous recordings of pupil size variation and locus coeruleus activity in mice

STAR Protocols
Summary: An extensive literature describes how pupil size reflects neuromodulatory activity, including the noradrenergic system. Here, we present a protocol for the simultaneous recording of optogenetically identified locus coeruleus (LC) units and pupil
Marine Megemont, Lucas S. Tortorelli, Jim McBurney-Lin, Jeremiah Y. Cohen, Daniel H. O’Connor, Hongdian Yang   +5 more
doaj   +1 more source

Application of a statistic tool for on-line characterization of bubble population complexity in a multiphase reactor [PDF]

, 2010
Este resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010.
Ferreira, A. M. A., Pereira, G., Rocha, F. A., Teixeira, J. A.   +3 more
core  

Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

FEBS Open Bio, EarlyView.
Induction of diabetes in three different mouse strains uniformly resulted in an increase in TNAP activity and a reduction in pyrophosphate (PPi) in the circulation. Inhibition of TNAP restored plasma PPi. Diabetes‐induced calcification in the media layer of the aorta was detected only in the Abcc6−/− strain, which is predisposed to ectopic ...
Krisztina Fülöp, Eszter Kozák, Natália Tőkési, Lilian Kocsis, Anikó Kelemen, Zsuzsanna Geszti, Adriána Kutás, Mariann Harangi, Ágnes Diószegi, Zsolt Rapi, József Balla, Olivier Le Saux, András Váradi, Viola Pomozi   +13 more
wiley   +1 more source

Protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes

STAR Protocols
Summary: The use of CRISPR-Cas9 ribonucleoproteins has revolutionized manipulation of genomes. Here, we present a protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes.
Fernando H. Biase, Gustavo Schettini
doaj   +1 more source

Calcium‐sensing receptor induces the apoptosis of chondrocytes in cooperation with phosphate transporter

FEBS Open Bio, EarlyView.
Excess Ca2+ ions activate the Calcium‐Sensing Receptor (CaSR), which subsequently drives the uptake of excess inorganic phosphate (Pi) via the Pi transporter (Pit−1) in chondrocytes. This mechanism causes a toxic increase in intracellular Pi concentration, ultimately leading to chondrocyte apoptosis and pathological mineralization. Excess extracellular
Sachie Nakatani, Hiroya Ueda, Ayumi Kawata, Yuki Sato, Kotomi Inomata, Hiroshi Mano, Masahiro Wada, Kenji Kobata   +7 more
wiley   +1 more source

A protocol for acquiring high-quality single-cell multi-omics data from human peripheral blood

STAR Protocols
Summary: Single-cell analysis of human peripheral blood cells provides insights into innate and adaptive immune systems. However, robust protocols are essential to ensuring single-cell sequencing data quality and cell viability.
Shanshan Duan, Guokang Ma, Junjie Chen, Xuyang Shi, Zishuo Yuan, Wenwen Zhou, Qiuting Deng, Yang Wang, Jianhua Yin, Yue Yuan, Chuanyu Liu   +10 more
doaj   +1 more source

Evolutionary dynamics of the chloroplast genome in Daphne (Thymelaeaceae): comparative analysis with related genera and insights into phylogenetics

FEBS Open Bio, EarlyView.
Comparative analysis of chloroplast genomes from 14 genera of Thymelaeaceae revealed variation in gene content, ranging from 128 to 142 genes, primarily influenced by IR expansion/contraction events and pseudogenization of ndhF, ndhI, and ndhG. Two large inversions were detected within the large single‐copy region, including a synapomorphic inversion ...
Abdullah, Hui Li, Rushan Yan, Chengyu Chen, Madiha Islam, Abdul Majid, Ibrar Ahmed, Parviz Heidari, Xiaoxuan Tian   +8 more
wiley   +1 more source

Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig

STAR Protocols
Summary: The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity
Yasmin Eckstein, Barbara Kessler, Arne Hinrichs, Istvan Novak, Anne von Thaden, Timo Lorenzen, Birgit Rathkolb, Armin Scholz, Andreas Blutke, Sietse-Jan Koopmans, Martin Hrabĕ de Angelis, Berit Østergaard Christoffersen, Eckhard Wolf, Simone Renner   +13 more
doaj   +1 more source

THE EFFECTS OF INFORMATION ON CONSUMER DEMAND FOR BIOTECH FOODS: EVIDENCE FROM EXPERIMENTAL AUCTIONS [PDF]

Consumers' willingness to pay for food products decreases when the food label indicates that a food product is produced with the aid of modern biotechnology.
Huffman, Wallace E., Rousu, Matthew C., Shogren, Jason F., Tegene, Abebayehu   +3 more
core   +1 more source