Results 121 to 130 of about 102,201 (258)

The impact of leukocyte‐associated RNA retention on platelet RNA‐based cancer detection

Molecular Oncology, EarlyView.
Platelets carry RNA signals that may help detect cancer, but platelet samples can also retain RNA from white blood cells. We found that this retained white blood cell signal did not significantly affect machine learning cancer classification, suggesting that the cancer‐related RNA signal in platelets is largely independent of leukocyte‐associated RNA ...
Michał Sieczczyński, Krzysztof Pastuszak, Anna Samelak‐Czajka, Zuzanna Cemka, Paulina Jackowiak, Tomasz Stefaniak, Sylwia Łapińska‐Szumczyk, Jacek Jassem, Anna J. Żaczek, Matthew T. Rondina, Myron G. Best, Sjors G. J. G. In 't Veld, Anna Supernat   +12 more
wiley   +1 more source

Protocol for minicircle production for gene therapy without subsequent cleanup steps

STAR Protocols
Summary: Current techniques for producing minicircles have low yields because of the cleanup steps required. Here, we present a protocol that can increase minicircle yield up to 10-fold by eliminating post-induction cleanup steps.
Jonathan Do, Gautam Verma, Yasmin Maurice, Muniba Abdumanobova, Zicheng Deng, Tanya V. Kalin, Vladimir V. Kalinichenko   +6 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

ADVANCES IN BIOTECHNOLOGY AND BIOENGINEERING: RESEARCH, TECHNIQUES, AND APPLICATIONS – VOLUME 6 2026 S1052B2

This volume integrates biotechnology with engineering principles to improve biological system design, biomaterial development, and industrial production efficiency. It highlights cellular engineering, biomolecular design, synthetic biology, biocatalysis,

core   +1 more source

1.4 Nature Conservation in the Age of Bioengineering [PDF]

Highlights how bioengineering could positively contribute to conservation efforts through thoughtful, collaborative, and ethical approaches.This entreaty was created as part of The Spirit of Asilomar and the Future of Biotechnology summit (February 23-26,
Freemont, Paul, Redford, Kent, Moronta-Barrios, Felix, Kiattisewee, Cholpisit, Seah, Adeline, Lindner, Ariel B., Novak, Ben   +6 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Protocol for engineering binding domains to recognize ligand-bound receptors by using yeast surface display

STAR Protocols
Summary: Yeast surface display is a versatile protein engineering technology, enabling precise control of the applied selection pressure. We present a yeast-surface-display-based protocol for the enrichment of binders specifically recognizing ligand ...
Markus Dobersberger, Delia Sumesgutner, Charlotte U. Zajc, Michael W. Traxlmayr   +3 more
doaj   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Protocol to visualize trans-interaction of clustered protocadherin using cIPAD, a fluorescent indicator, in cultured human cells and mouse neurons

STAR Protocols
Summary: cIPAD is a fluorescent indicator that allows the visualization of trans-interactions of clustered protocadherin (Pcdh), a cell adhesion molecule that mediates neuronal self-recognition. We describe steps for using HEK293T cells to visualize Pcdh
Takashi Kanadome, Natsumi Hoshino, Takeharu Nagai, Takeshi Yagi, Tomoki Matsuda   +4 more
doaj   +1 more source

Current Developments in Biotechnology and Bioengineering: Resource Recovery from Wastes

Current Developments in Biotechnology and Bioengineering: Resource Recovery from Wastes includes the latest and innovative research and technological developments in the biotechnology and bioengineering pertaining to various resource(s) recovery from ...
Varjani, Sunita, Pandey, Ashok, Khanal, Samir Kumar, Raveendran, Sindhu, Gnansounou, Edgard   +4 more
core