Results 201 to 210 of about 456,411 (294)
Turbo-RIP: A Protocol for TurboID-based RNA Immunopurification to Map RNA Landscapes in Plant Biomolecular Condensates. [PDF]
Zhang Z, Xu Y, Liu H, Liu C, Moschou PN.
europepmc +1 more source
OsGSK2‐OsTCP19 module regulating nitrate response and lateral root (LR) development. Low‐nitrate condition results in reduced BR response, accumulation of OsGSK2 and phosphorylated OsTCP19, which suppresses the expression of nitrate‐responsive genes and LR‐development genes and impairs rice growth.
Yongqiang Liu +11 more
wiley +1 more source
Enantioselective electrophilic α-fluorination catalyzed by an artificial metalloenzyme. [PDF]
Yu J +5 more
europepmc +1 more source
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley +1 more source
Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis
In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
wiley +1 more source
Features and mechanism of localized enzyme-assisted self-assembly of peptides from unilamellar vesicles. [PDF]
Ontani A +7 more
europepmc +1 more source
Magnetomechanical neuromodulation using magnetic nanodiscs enables remote activation of neurons. In a hemiparkinsonian mouse model, alternating magnetic fields actuate the nanodiscs to generate torque that opens mechanosensitive ion channels within the subthalamic nucleus, thereby modulating basal ganglia motor circuitry.
Anouk Wolters +12 more
wiley +1 more source
Enhancing the sensitivity of nanobodies through covalent and non-covalent polymerization. [PDF]
Wang F +5 more
europepmc +1 more source

