Results 271 to 280 of about 54,144 (311)
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Molecular Immunology, 1982
Purified human C3 was biotinylated using the biotinyl-N-hydroxysuccinimide imidoester (BNHS). Depending on the input of BNHS, from three to six molecules of biotin were incorporated per C3 molecule. The biotinyl-C3 retained over 90% of its specific hemolytic activity and when bound to sheep erythrocytes maintained its ability to adhere to human C3b ...
M, Berger +5 more
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Purified human C3 was biotinylated using the biotinyl-N-hydroxysuccinimide imidoester (BNHS). Depending on the input of BNHS, from three to six molecules of biotin were incorporated per C3 molecule. The biotinyl-C3 retained over 90% of its specific hemolytic activity and when bound to sheep erythrocytes maintained its ability to adhere to human C3b ...
M, Berger +5 more
openaire +3 more sources
The Journal of Organic Chemistry, 2004
Inhibition of the 90 kDa heat shock proteins (Hsp90) represents a promising new chemotherapeutic approach for the treatment of several cancers. Hsp90 is essential to the survival of cancer cells and is inhibited by members of the ansamycin family of antibiotics.
Randell C, Clevenger +3 more
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Inhibition of the 90 kDa heat shock proteins (Hsp90) represents a promising new chemotherapeutic approach for the treatment of several cancers. Hsp90 is essential to the survival of cancer cells and is inhibited by members of the ansamycin family of antibiotics.
Randell C, Clevenger +3 more
openaire +2 more sources
Biotinylated Stealth® magnetoliposomes
Chemistry and Physics of Lipids, 2002Dimyristoylphosphatidylethanolamine (DC(14:0)PE) and the dioleoyl analogue (DC(18:1cis)PE) were mixed with alpha-biotinylamido-omega-N-succinimidoxycarbonyl-poly(ethylene glycol) (NHS-PEG-biotin) and quantitatively converted to alpha-biotinylamido-omega-(dimyristoylphosphatidylethanolamino-carbonyl)polyethylene glycol (DC(14:0)PE-PEG-biotin) and the ...
Michael, Hodenius +5 more
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Rapid Enzyme-Mediated Biotinylation for Cell Surface Proteome Profiling.
Analytical Chemistry, 2021Cell surface is the primary site for sensing extracellular stimuli. The knowledge of the transient changes on the surfaceome upon a perturbation is very important as the initial changed proteins could be driving molecules for some phenotype.
Yanan Li +7 more
semanticscholar +1 more source
Directly Quantifiable Biotinylation Using a Water-Soluble Isatoic Anhydride Platform.
Bioconjugate chemistry, 2021Isatoic anhydride (IA) has been shown to be a useful platform for quantifiable bioconjugation. The elaboration of a water-soluble isatoic anhydride-based platform with biotin offers readily quantifiable biotinylation reagents through nondestructive ...
Adam B. Fessler, A. Fowler, C. Ogle
semanticscholar +1 more source
Synthesis of biotinylated diazinon: Lessons learned for biotinylation of thiophosphate esters
Tetrahedron Letters, 2018Abstract Biotinylation permits recovery of a molecule from a complex mixture, with commercially available streptavidin containing products (such as streptavidin-coated beads). As part of a larger effort to evaluate reagents capable of degrading diazinon, a thiophosphate insecticide, we pursued biotinylation of this molecule.
Kyle G. Nottingham +2 more
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Current Protocols in Protein Science, 2010
AbstractSince its discovery in the first half of the twentieth century, the high‐affinity, noncovalent interaction between biotin (vitamin H) and the avian protein avidin (and its bacterial homologs) has been exploited for many diverse biotechnology applications.
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AbstractSince its discovery in the first half of the twentieth century, the high‐affinity, noncovalent interaction between biotin (vitamin H) and the avian protein avidin (and its bacterial homologs) has been exploited for many diverse biotechnology applications.
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Fragmentation of biotinylated cyclic peptides
Rapid Communications in Mass Spectrometry, 2004Abstract Electrospray ionization coupled with tandem mass spectrometry (MS/MS) was used to determine the preferred binding site(s) of biotin NHS ester with a series of cyclic peptides with antibiotic properties.
Michael E, Lassman +2 more
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Biotinylated gene therapy vectors
Expert Opinion on Biological Therapy, 2003The avidin-biotin system is a fundamental technology in biomedicine for immunolocalisation, imaging, nucleic acid blotting and protein labelling. This technology has recently been adapted for use in gene therapy vector applications to add proteins or cell-targeting ligands to non-viral and viral vectors. Two biotinylation technologies are being used in
Michael A, Barry +6 more
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In vivo enzymatic protein biotinylation
Biomolecular Engineering, 1999Biotin is biologically active only when protein-bound and is covalently attached to a class of important metabolic enzymes, the biotin carboxylases and decarboxylases. Biotinylation is a relatively rare modification, with between one and five biotinylated protein species found in different organisms.
A, Chapman-Smith, J E, Cronan
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