Results 281 to 290 of about 54,144 (311)

Biotinylation of Antibodies

1994
Using the characteristic of a high-affinity complex between avidin and biotin, biotinylated antibodies have wide applications in various immunochemical assays, especially where signal amplification is required. A method is described here for the biotinylation of immunoglobulins.
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Protein Biotinylation

2014
Biotin is a naturally occurring vitamin that binds with high affinity to avidin and streptavidin proteins. Because biotin is small (244 Da), it can be conjugated to many proteins without altering their biological activities. The biotinylated molecule can be detected in ELISA, dot blot, or Western blot methods (see Western Blotting using ...
openaire   +2 more sources

Effective Methods for the Biotinylation of Azamacrocycles

The Journal of Organic Chemistry, 2007
The biotin-(strept)avidin interaction remains a gold standard of model biological recognition events. The biotinylation of azamacrocycles permits the investigation of signal transduction between this recognition event and the metal center of an azamacrocycle complex, of wide potential interest in biosensing. There are no generally applicable procedures
Sara J, Krivickas, Emiliano, Tamanini, Matthew H, Todd, Michael, Watkinson   +3 more
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Affinity chromatography with biotinylated RNAs

1990
Small, reversibly biotinylated RNAs as described here are versatile ligands for affinity chromatography of RNA-binding components. These RNAs can be attached to a solid support by binding to avidin and used as ligands, or they may be hybridized to another RNA which acts as the ligand.
S W, Ruby, S E, Goelz, Z, Hostomsky, J N, Abelson   +3 more
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4‐Thiouridine‐Enhanced Peroxidase‐Generated Biotinylation of RNA

ChemBioChem, 2020
Peroxidase‐generated proximity labeling is in widespread use to study subcellular proteomes and the protein interaction networks in living cells, but the development of subcellular RNA labeling is limited.
Jinguo Huang, Ruiqi Zhao, Shan-shan Qin, Shixi Yang, Wei Li, Jing Mo, F. Wang, Yuhao Du, X. Weng, Xiang Zhou   +9 more
semanticscholar   +1 more source

Syntheses of biotinylated and dethiobiotinylated insulins

Biochemistry, 1984
The 600-MHz proton spectrum of dethiobiotin (prepared from d-biotin with Raney nickel) was measured in order to gain information pertaining to its stereochemical homogeneity. The spectrum demonstrated clearly that the material is a 6:1 mixture of two stereoisomers. The cis compound, corresponding to the stereochemistry of d-biotin, is the major isomer.
K, Hofmann, W J, Zhang, H, Romovacek, F M, Finn, A A, Bothner-By, P K, Mishra   +5 more
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A proximity-dependent biotinylation map of a human cell

Nature, 2021
Christopher D. Go, Christopher D. Go, J. Knight, Archita Rajasekharan, B. Rathod, G. Hesketh, K. Abe, K. Abe, J. Youn, Payman Samavarchi-Tehrani, Hui Zhang, Lucie Y Zhu, Evelyn Popiel, Jean-Philippe Lambert, Jean-Philippe Lambert, É. Coyaud, É. Coyaud, Sally W T Cheung, D. Rajendran, Cassandra J. Wong, H. Antonicka, L. Pelletier, L. Pelletier, B. Raught, B. Raught, Alexander F. Palazzo, E. Shoubridge, A. Gingras, A. Gingras   +28 more
semanticscholar   +1 more source

Solid‐phase biotinylation of antibodies

Journal of Molecular Recognition, 2004
AbstractBiotinylation is an established method of labeling antibody molecules for several applications in life science research. Antibody functional groups such as amines, cis hydroxyls in carbohydrates or sulfhydryls may be modified with a variety of biotinylation reagents.
Elizabeth, Strachan, A Krishna, Mallia, Joanna M, Cox, Babu, Antharavally, Surbhi, Desai, Laura, Sykaluk, Valerie, O'Sullivan, Peter A, Bell   +7 more
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Hybrid Selection of mRNA with Biotinylated DNA

Gene Analysis Techniques, 1990
A novel, convenient, and highly efficient hybrid selection procedure is described. The method utilizes the polymerase chain reaction (PCR) in which one of two primers is biotinylated at the 5'-terminus. The concentration of the biotinylated primer is 100 times that of the other to synthesize biotinylated single-stranded DNA (asymmetric PCR).
J, Soh, S, Pestka
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Biotinylated Probes in Colony Hybridization

1998
Colony hybridization is a procedure that allows the detection of cells containing nucleic acid sequences of interest (1). In this method, microbial colonies grown on, or transferred to, a supporting membrane are lysed and their nucleic adds denatured to single strands and fixed in place on the membrane.
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