Results 291 to 300 of about 54,144 (311)

Site‐specific biotinylation

International Journal of Peptide and Protein Research, 1992
We have developed an expeditious method for the incorporation of the biotinylaminocaproyl moiety on the ε‐amino group of a lysine residue within a peptide chain in a site‐specific manner. Using t‐Boc chemistry for the solid phase synthesis approach and a base labile, acid stable protecting group (Fmoc‐) for the ε‐amino group of the target lysine, we ...
SESHA NATARAJAN, STEPHEN M. FESTIN, ANDERS HEDBERG, EDDIE C.‐K. LIU, DAVID M. FLOYD, JOHN T. HUNT   +5 more
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[14] Protein biotinylation

1990
Publisher Summary This chapter discusses the procedures by which researchers have incorporated biotin moieties into proteins. Several laboratories have reported the use of an avidin-based assay for detecting the extent of biotinylation. An effective method for detecting the average number of biotin groups per protein molecule would be to first remove
Edward A. Bayer, Meir Wilchek
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Biotinylated peptides/proteins. II. Identification of biotinylated lysyl phenylthiohydantoins.

Analytical biochemistry, 1991
The identification and characterization of biotinylated lysyl residues in a polypeptide chain by automated sequence analysis is described. An in depth analytical study was conducted for the delineation of N epsilon modification of lysyl residues with N-hydroxysuccinimide esters of biotin and 6-aminohexanoic biotin.
J S, Smith, B T, Miller, A, Kurosky
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Selections on Biotinylated Antigens

2001
Phage antibody library selections on peptides or proteins are usually carried out using antigens directly coated on a plastic surface (e.g. Petri dishes, microtiter plate well, immunotubes, Chapter 9). This straightforward method is easy to perform and has been shown to be very successful for a diverse set of antigens (for review see Winter et al. 1994)
Patrick Chames, Hennie R. Hoogenboom, Paula Henderikx   +2 more
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Reversible biotinylation of C1q with a cleavable biotinyl derivative

Journal of Immunological Methods, 1988
Berhane Ghebrehiwet, Steven Bossone, Anna Erdei, Kenneth B.M. Reid   +3 more
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Getting to know the neighborhood: using proximity-dependent biotinylation to characterize protein complexes and map organelles.

Current Opinion in Chemical Biology, 2019
A. Gingras, K. Abe, B. Raught
semanticscholar   +1 more source

Avidin binding of biotinylated corticotropins

Biochemistry, 1983
H, Romovacek, F M, Finn, K, Hofmann
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Targeting Applications of Biotinylated Liposomes

Journal of Liposome Research, 1993
M. Soria, H. Loughrey, A. Ferraretto, A. M. Cannon, G. Acerbis, F. Sudati, G. Bottiroli, M. Masserini   +7 more
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Biotinylation (Biotinylated)

2008
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Kinase-Catalyzed Biotinylation.

Current protocols in chemical biology
Kinase-catalyzed protein phosphorylation plays an essential role in a variety of biological processes. Methods to detect phosphoproteins and phosphopeptides in cellular mixtures will aid in cell biological and signaling research. Our laboratory recently discovered the utility of γ-modified ATP analogues as tools for studying phosphorylation ...
Chamara, Senevirathne, Keith D, Green, Mary Kay H, Pflum   +2 more
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