Results 151 to 160 of about 20,884 (264)

Introduction of a Double‐Headed Nucleotide Into G‐Quadruplex DNA: Position‐Dependent Stabilization and Structural Insights

open access: yesChemistry – A European Journal, EarlyView.
A double‐headed bis‐guanine (G) nucleotide (GG) was evaluated as a noncanonical building block in G‐quadruplexes (G4s). Biophysical and computational analyses reveal that GG can directly participate in G‐tetrads and strongly stabilize G4s in a position‐ and topology‐dependent manner. These findings establish design rules for using GG to engineer highly
Krista Urup   +5 more
wiley   +1 more source

Engineering and Characterization of a Fluorescent Fission Yeast Arp2/3 Complex for Single Molecule Mechanistic Investigations

open access: yesCytoskeleton, EarlyView.
ABSTRACT Arp2/3 complex is a seven‐component protein complex that facilitates the assembly of branched actin filament networks by binding to pre‐existing “mother” actin filaments and initiating the nucleation of new branched “daughter” filaments. Arp2/3 complex must be activated by a nucleation promoting factor such as the WASP/Scar protein family. The
Caitlin A. Anderson   +8 more
wiley   +1 more source

Classroom boundaries and teacher agency: Challenges of implementing Ireland's new primary curriculum

open access: yesThe Curriculum Journal, EarlyView.
Abstract This article reports on a doctoral study examining teacher agency in one Irish primary school at a timely moment ahead of the implementation of the new Primary Curriculum Framework in September 2025. The framework embeds teacher agency as a central professional principle, yet findings from this study reveal a more cautious and bounded reality.
Máiréad Nally   +2 more
wiley   +1 more source

Upper bound limit analysis of coral reef limestone cavern roof stability incorporating a tension‐shear failure mechanism with tensile‐strength cut‐off

open access: yesDeep Underground Science and Engineering, EarlyView.
For the roof of coral reef limestone caverns, a novel tension‐shear composite failure mechanism was developed. The most critical tensile crack model was identified using a hybrid optimization algorithm, and the stability of the cavern roof was analyzed accordingly.
Dongsheng Xu, Chenxu Li, Chuantan Hou
wiley   +1 more source

Organizational Tensions in the Implementation of Modifiable Off-the-Shelf Technologies in a University Hospital: Qualitative Multimethod Study. [PDF]

open access: yesJMIR Hum Factors
Nowak A   +12 more
europepmc   +1 more source

Oogenesis and germinal bed morphology of the brown anole (A. sagrei)

open access: yesDevelopmental Dynamics, EarlyView.
Abstract Background The brown anole is a model species of the genus Anolis, a squamate (encompassing lizards and snakes) group widely studied in evolutionary, behavioral, and developmental biology. Full genome annotation, the establishment of gene editing techniques, and comprehensive description of reproductive tract morphology and embryogenesis in ...
Bonnie K. Kircher   +12 more
wiley   +1 more source

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