Results 121 to 130 of about 15,618,864 (298)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

open access: yesFEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata   +13 more
wiley   +1 more source

Gobies on the Turkish coast of the Black Sea, and first record of the yellow-headed goby, Gobius xanthocephalus Heymer & Zander, 1992

open access: yesTurkish Journal of Bioscience and Collections
Objective: Challenges in identifying species of the Gobiidae family and their high tolerance to salinity, which makes it difficult to determine their distribution boundaries, have prevented the achievement of a common checklist for these species in the ...
Nur Bikem Kesici, Cem Dalyan
doaj   +1 more source

Editorial Foreword [PDF]

open access: yesRevista Română pentru Studii Baltice şi Nordice, 2014
The current issue of Revista Română de Studii Baltice şi Nordice / The Romanian Journal for Baltic and Nordic Studies gathers in its first part the syllabi of the courses taught during the Romanian summer school of Nordic and Baltic studies entitled “A ...
Crina Leon
doaj  

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

open access: yesFEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura   +19 more
wiley   +1 more source

Nitrogen Problem for the Black Sea Ecosystem

open access: yes, 2013
The aim of the report presented was development of a comprehensive scheme of balance and biogeochemical N cycling for the Black Sea: discussion of exchange of N, its main sources/sinks and the processes involved: identify weak under-studied points and ...
Medinets, Sergiy V.
core   +1 more source

Spatial Variation and Uncertainty Analysis of Black Sea Level Change from Virtual Altimetry Stations over 1993–2020

open access: yesRemote Sensing
Global mean sea level has been rising steadily since the early 1990s, yet regional sea level changes exhibit complex spatial variability that frequently contrasts with global trends.
Yuxuan Fan   +6 more
doaj   +1 more source

NATURAL RESOURCES AS POWER INCREASNG CAPABILITIES AFFECTING THE POLARITY OF THE BLACK SEA REGIONAL SYSTEM [PDF]

open access: yesEURINT, 2016
Building on the neorealist assumption, the current study argues that during the analysed time frame (2006-2016), the energy related issues have contributed to a shift in the Black Sea regional structure which drastically affected the regional balance of ...
Oana-Ancuța POIANĂ
doaj  

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