Results 111 to 120 of about 15,619,244 (298)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Revista Română pentru Studii Baltice şi Nordice, 2014 The current issue of Revista Română de Studii Baltice şi Nordice / The Romanian Journal for Baltic and Nordic Studies gathers in its first part the syllabi of the courses taught during the Romanian summer school of Nordic and Baltic studies entitled “A ...
Crina Leon
doaj
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Turkish Journal of Bioscience and CollectionsObjective: Challenges in identifying species of the Gobiidae family and their high tolerance to salinity, which makes it difficult to determine their distribution boundaries, have prevented the achievement of a common checklist for these species in the ...
Nur Bikem Kesici, Cem Dalyan
doaj +1 more source
FEBS Open Bio, EarlyView.Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
Nitrogen Problem for the Black Sea Ecosystem
, 2013 The aim of the report presented was development of a comprehensive scheme of balance and biogeochemical N cycling for the Black Sea: discussion of exchange of N, its main sources/sinks and the processes involved: identify weak under-studied points and ...
Medinets, Sergiy V.
core +1 more source
Remote SensingGlobal mean sea level has been rising steadily since the early 1990s, yet regional sea level changes exhibit complex spatial variability that frequently contrasts with global trends.
Yuxuan Fan +6 more
doaj +1 more source
NATURAL RESOURCES AS POWER INCREASNG CAPABILITIES AFFECTING THE POLARITY OF THE BLACK SEA REGIONAL SYSTEM [PDF]
EURINT, 2016 Building on the neorealist assumption, the current study argues that during the analysed time frame (2006-2016), the energy related issues have contributed to a shift in the Black Sea regional structure which drastically affected the regional balance of ...
Oana-Ancuța POIANĂ
doaj