Results 141 to 150 of about 4,554 (244)

Protocol for the isolation of human buccal cells for single-cell applications. [PDF]

STAR Protoc
Buranapraditkun S, Rodsaward P, Chottawornsak N, Thantiworasit P, Wongpiyabovorn J, Chantaphakul H.   +5 more
europepmc   +1 more source

Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning

Cytoskeleton, EarlyView.
ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham, Mihir Relan, Matthew T. Swulius, Andrea K. H. Stavoe   +3 more
wiley   +1 more source

Surgical protocol for precise and high-throughput viral injections in rhesus monkey brain. [PDF]

STAR Protoc
Tangen AC, Harmon P, Plotnikova AS, Mohanty A, Cummins AC, Pelkey KA, Cameron K, Rood C, Guerriero KA, McBain CJ, Eldridge MAG, Averbeck BB, Azadi R.   +12 more
europepmc   +1 more source

Double Sided Traction Force Microscopy: A Method to Confine Cells for Physiologically Relevant Force Measurements

Cytoskeleton, EarlyView.
ABSTRACT In this paper we describe a technique to make a confined environment of variable stiffness that is suitable for high‐resolution live‐cell imaging. This versatile and adaptable technique enables cell confinement between soft elastic surfaces made from polyacrylamide gels.
Alexia Caillier, Patrick W. Oakes
wiley   +1 more source

Airway Management With a GlideScope® Spectrum LoPro Blade in a Child With Giant Facial Rhabdomyosarcoma: A Case Report. [PDF]

Case Rep Anesthesiol
Kosokabe M, Sunaga S, Sekigawa A, Niiyama Y.   +3 more
europepmc   +1 more source

Comparison of Tissue Preparations to Identify and Phenotype T Cells in Human Colorectal Tumor Tissue

Cytometry Part A, EarlyView.
ABSTRACT Archived patient samples (e.g., formalin‐fixed paraffin embedded (FFPE) tissue blocks, cryoembedded tissue blocks, and frozen whole tissue samples) are a source of immune information for which corresponding patient data is often collected.
Justin F. Tirados, Sonya Fenton, Helen M. McGuire, Joanna M. Roberts, Kirsten A. Ward‐Hartstonge, Roslyn A. Kemp   +5 more
wiley   +1 more source

Measuring Electrophysiological Activity in Acute Brain Slices, Spheroids, and Organoids Using 3D High-Density Multielectrode Arrays. [PDF]

Bio Protoc
Pali E, Pellavio G, Conforti M, Sarkissian AA, Aliya B, Sciacca G, Wariyar SS, Mainardi F, Tedesco M, Verduci I, Cadiao G, Cervetto C, Andersen J, Birey F, Maccione A, D'Angelo E, Mapelli L.   +16 more
europepmc   +1 more source

Controlled Nanoarchitecture of Aligned Electrospinning Fiber Composite Electrolyte Based on Plant and Animal Biopolymers for Safe Na‐Ion Batteries

ENERGY &ENVIRONMENTAL MATERIALS, EarlyView.
Aligned Electrospun Composite Electrolyte. The resurgence of solid‐state Na+ battery, sustained through lithium deficiency and mounting costs, offers an auspicious alternative to solid‐state Li+ battery. This study introduces a nanoarchitectural approach that integrates electrospinning with a simple solution casting technique to develop a mechanically ...
Md. Mehadi Hassan, Brett Conners, Mojtaba Ebrahimian Mashhadi, Jinguang Hu, Xia Li, Qingye Lu   +5 more
wiley   +1 more source

Mechanical Properties and Parameter Optimization for the "Suitable Harvest" Stage of Vegetable Sweet Potato Shoot Tips in Mechanized Harvesting. [PDF]

Plants (Basel)
Shen H, Qiao O, Wang G, Xue G, Ding W, Hu L, Zhou G.   +6 more
europepmc   +1 more source

Flexible Direct‐Conversion X‐Ray Detectors: From Emerging Materials to Conformal Imaging

ENERGY &ENVIRONMENTAL MATERIALS, EarlyView.
This comprehensive review analyzed the performance, challenges, and future directions of four emerging material systems for next‐generation flexible direct‐conversion X‐ray detectors: organic semiconductors, perovskites, metal–organic frameworks, and inorganic metal compounds.
Haipeng Di, Guoliang Ma, Jiaxin Mu, Kailian Dong, Haibin Li, Manjing Wang, Xin Song, Haojin Li, Jianwei Xu, Yiying Zhao, Jingjing Zhao, Guojia Fang   +11 more
wiley   +1 more source