Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Prediction of spallation induced transmutation rates for long-lived fission products via proton accelerator. [PDF]
Tukharyan G +4 more
europepmc +1 more source
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Process Temperature Control for Low Dishing in CMP. [PDF]
Shin Y, Jeong J, Shin J, Jeong H.
europepmc +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
"Three-Color" Chemical Selectivity between Oxide, Nitride, and Silicon: Area-Selective Deposition of Metal Oxide and Polymer on SiN and Si-H versus SiO<sub>2</sub>. [PDF]
Thelven JM +6 more
europepmc +1 more source
Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar +4 more
wiley +1 more source
Standardizing Perioperative Heat to Improve Mouse (Mus musculus) Recovery. [PDF]
Popadich AJ +7 more
europepmc +1 more source
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura +19 more
wiley +1 more source
Selective Etching of Multi-Stacked Epitaxial Si<sub>1-x</sub>Ge<sub>x</sub> on Si Using CF<sub>4</sub>/N<sub>2</sub> and CF<sub>4</sub>/O<sub>2</sub> Plasma Chemistries for 3D Device Applications. [PDF]
Kim J, Kang J, Yoon D, Chung UI, Ko DH.
europepmc +1 more source

