Results 31 to 40 of about 69,846 (258)

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Environmental Drivers of Variation in Bleaching Severity of Acropora Species during an Extreme Thermal Anomaly

Frontiers in Marine Science, 2017
High sea surface temperatures caused global coral bleaching during 2015–2016. During this thermal stress event, we quantified within- and among-species variability in bleaching severity for critical habitat-forming Acropora corals.
Mia O. Hoogenboom, Mia O. Hoogenboom, Grace E. Frank, Grace E. Frank, Tory J. Chase, Tory J. Chase, Saskia Jurriaans, Saskia Jurriaans, Mariana Álvarez-Noriega, Mariana Álvarez-Noriega, Katie Peterson, Kay Critchell, Kathryn L. E. Berry, Kathryn L. E. Berry, Kathryn L. E. Berry, Katia J. Nicolet, Katia J. Nicolet, Blake Ramsby, Blake Ramsby, Allison S. Paley, Allison S. Paley   +20 more
doaj   +1 more source

Combination Analysis of Metatranscriptome and Metagenome Reveal the Composition and Functional Response of Coral Symbionts to Bleaching During an El Niño Event

Frontiers in Microbiology, 2020
With the abnormal rise in ocean temperatures globally in recent years, coral bleaching is becoming common and serious. However, the response mechanisms and processes of coral symbionts to bleaching are not well understood. In this study, metagenomics and
Fulin Sun, Fulin Sun, Fulin Sun, Fulin Sun, Hongqiang Yang, Hongqiang Yang, Hongqiang Yang, Hongqiang Yang, Guan Wang, Guan Wang, Guan Wang, Qi Shi, Qi Shi, Qi Shi   +13 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

PROCESS OPTIMIZATION OF TETRA ACETYL ETHYLENE DIAMINE ACTIVATED HYDROGEN PEROXIDE BLEACHING OF POPULUS NIGRA CTMP

BioResources, 2010
To enhance the bleaching efficiency, the activator of tetra acetyl ethylene diamine (TAED) was used in conventional H2O2 bleaching. The H2O2/TAED bleaching system can accelerate the reaction rate and shorten bleaching time at relative low temperature ...
Qiang Zhao, Junwen Pu, Shulei Mao, Guibo Qi   +3 more
doaj  

Surface Damage and Bleaching Effect according to the Application Type of Home Tooth Bleaching Applicants

치위생과학회지, 2020
Background: In this study, the bleaching effect and surface damage of two types of over-the-counter home tooth bleaching agents were explored using an in vitro study of bleaching agents applied to bovine teeth specimens for 14 days.Methods: Domestic over-
Na-Yeoun Tak, Do-Seon Lim, Hee-Jung Lim, Im-Hee Jung   +3 more
doaj   +1 more source

Comparison of Casein Phosphopeptide with Potassium Nitrate and Sodium Monofluorophosphate Desensitizing Efficacy after In-Office Vital Bleaching—A Randomized Trial

Applied Sciences, 2021
Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), potassium nitrate, and sodium monofluorophosphate are desensitizing agents that help in reducing teeth sensitivity. However, indecisive evidence exists regarding their efficacy. Therefore, this
Hafiz Muhammad Adil, Rizwan Jouhar, Muhammad Adeel Ahmed, Sakeenabi Basha, Naseer Ahmed, Maria Shakoor Abbasi, Afsheen Maqsood, Anil Kumar Nagarajappa, Mohammad Khursheed Alam   +8 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source