Results 141 to 150 of about 1,246,514 (354)

Construction of hyperthermostable d‐allulose 3‐epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus

open access: yesFEBS Open Bio, EarlyView.
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada   +3 more
wiley   +1 more source

Determination of fluphenazine, related phenothiazine drugs and metabolites by combined high-performance liquid chromatography and radioimmunoassay. [PDF]

open access: yes, 1981
Antibodies have been produced in rabbits immunized with a fluphenazine succinate-human serum albumin conjugate. By radioimmunoassay it is possible to quantify fluphenazine (FPZ), related phenothiazine drugs and several of their metabolites at the ...
Goldstein, SA, Van Vunakis, H
core  

Current trends in single‐cell RNA sequencing applications in diabetes mellitus

open access: yesFEBS Open Bio, EarlyView.
Single‐cell RNA sequencing is a powerful approach to decipher the cellular and molecular landscape at a single‐cell resolution. The rapid development of this technology has led to a wide range of applications, including the detection of cellular and molecular mechanisms and the identification and introduction of novel potential diagnostic and ...
Seyed Sajjad Zadian   +6 more
wiley   +1 more source

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

open access: yesFEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo   +4 more
wiley   +1 more source

Preparation and characterization of renal cell peptides from fetal rats for their antitumor activity

open access: yesFEBS Open Bio, EarlyView.
This study aimed to prepare renal cells (RCs) from fetal rats which were digested by enzymes. Candidate peptides RCPs were characterized by capillary HPLC and MS and their bioactivity was predicted using peptideranker. The predicted top 10 bioactive peptides were synthesized.
Zhe Zhang   +6 more
wiley   +1 more source

THE IMPORTANCE OF THE SAMPLING AND ERROR SOURCES OF THE RED CELL SEDIMENTATION RATE FOR DENTAL MEDICINE

open access: yesRomanian Journal of Oral Rehabilitation
Background and objectives: Erythrocyte sedimentation rate test guides dentists in the suspicion of inflammatory reactions or infections of the dental focus.
Loredana Liliana Hurjui   +7 more
doaj   +1 more source

Characterization of ribosome heterogeneity during endothelial to hematopoietic transition

open access: yesFEBS Open Bio, EarlyView.
The panorama of ribosome heterogeneity during embryonic hematopoiesis has not yet been portrayed. In this study, utilizing dual‐omics data, the heterogenous dynamic of ribosome during endothelial‐to‐hematopoietic transition has been systemically described. Moreover, stage‐specific upregulation and peripheral localization of RPL27 and RACK1 in hemogenic
Xitong Tian   +4 more
wiley   +1 more source

Concentrations of sodium 3α, 7α--dihydroxy-12-oxo 5β cholanate in biological material after its intravenous and intranasal application [PDF]

open access: yesPraxis Medica, 2018
Newly synthetized derivative of bile acid, sodium salto f 3 α, 7 α-dihydroxy-12-oxo 5β cholanic acid (monoketocholanate) expressed a good characteristic as intranasal transport enhancer of xenobiotics.
Stević Snežana   +4 more
doaj  

Determination of antimicrobial susceptibilities on infected urines without isolation [PDF]

open access: yes, 1979
A method is described for the quick determination of the susceptibilities of various unidentified bacteria contained in an aqueous physiological fluid sample, particularly urine, to one or more antibiotics.
Barza, M. J.   +6 more
core   +1 more source

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