Results 91 to 100 of about 314,842 (260)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Quantitative Histomorphometric Analysis of Collagen Bundles in Masson's Trichrome Stained Rat (Rattus norvegicus) Skin: A Methodological Study

Health Science Reports
Background and Aims While Masson's Trichrome is widely used for collagen visualization, its qualitative nature limits reproducibility and statistical comparison.
Okky Husain, Teresa Liliana Wargasetia, Julia Windi Gunadi, Angkasa Ramatuan Hamdan   +3 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

ENVIRONMENTALLY FRIENDLY TEMPORARY ANTI-MOULD TREATMENT OF PACKAGING MATERIAL BEFORE DRYING

Maderas: Ciencia y Tecnología, 2008
According to FAO - ISPM No 15, in international trade wood packaging material on the basis of sawn timber requires to be heat-treated to avoid spreading of harmful organisms.
Johannes Welling, Gerda Lambertz
doaj  

A New Lignin-Based Polyurethane Film for Wood: Decay, Artificial Weathering, Physical and Morphological Characterization

Molecules
Lignin-based polyurethanes represent a promising strategy for developing more sustainable wood coatings by partially replacing fossil-derived polyols with renewable aromatic biopolymers.
Swati Tamantini, Sara Bergamasco, Miha Humar, Marko Petrič, Manuela Romagnoli   +4 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Influencia del azulado (mancha azul) en la densidad y estabilidad dimensional de la madera de Pinus sylvestris Influence of blue stain on density and dimensional stability of Pinus sylvestris timber

Maderas: Ciencia y Tecnología, 2012
La madera de Pinus sylvestris tiene interés comercial aunque su valor se reduce considerablemente cuando presenta hongos de azulado. En el presente estudio se apearon siete pinos procedentes de plantaciones españolas, y de los fustes se muestrearon 14 ...
Adrián Rey-Prieto, Guillermo Riesco-Muñoz   +1 more
doaj  

Dynamics of wood decay in the cross-section of logs as a result of long-term storage in a landing yard

Drewno
Under investigation three deadwood landing yards were established - two with pine wood and one with spruce wood. Three measuring baskets were placed on each landing yard. In each basket round timbers were arranged in three test layers. In each test layer,
Krzysztof Michalec, Janusz Sowa, Radosław Wąsik, Monika Gach, Dariusz Kulak, Arkadiusz Stańczykiewicz, Anna Barszcz, Łukasz Mateusiak   +7 more
doaj   +1 more source

A Comparative Study of Mast Cells Count in Different Histological Grades of Oral Squamous Cell Carcinoma by Using Toluidine Blue Stain. [PDF]

Cureus, 2020
Narayan KV, Sonia G, Shrestha P, Hemadala G.   +3 more
europepmc   +1 more source