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Demonstration of antibodies against bovine leukemia virus (BLV) by blocking ELISA using bovine polyclonal anti-BLV immunoglobulin

Veterinary Microbiology, 1991
A blocking enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against bovine leukemia virus (BLV) is described. The test is based on the biotin-streptavidin system using unlabelled polyclonal bovine IgG against BLV as catching antibody and biotinylated bovine anti-BLV IgG as detecting antibody.
P. Have, Rikke Hoff-Jørgensen
openaire   +3 more sources

Recent studies on the characterization of the bovine leukemia virus (BLV); development of new methods for the diagnosis of BLV infection

Veterinary Microbiology, 1976
Abstract In this report data presented show that the bovine leukemia virus (BLV) is infectious for cells of various species including humans. Bat monolayer cell cultures were the most permissive for replication of BLV and provided an abundant source of virus for characterization studies.
C. Diglio   +7 more
openaire   +2 more sources

Augmentation of Bovine Leukemia Virus (BLV)‐Specific Lymphocyte Proliferation Responses in Ruminants by Inoculation with BLVenv‐Recombinant Vaccinia Virus: Their Role in the Suppression of BLV Replication

Microbiology and Immunology, 1992
AbstractLymphocyte proliferation responses were investigated in sheep and cattle, in which the replication of bovine leukemia virus (BLV) had been known to be suppressed by inoculation with recombinant vaccinia virus (rVV) expressing BLV envelope glycoprotein (gp60).
Masanobu Sugimoto   +8 more
openaire   +3 more sources

The BLV-induced leukemia-lymphosarcoma complex in sheep

Veterinary Immunology and Immunopathology, 1989
Sheep are highly susceptible to BLV infection and can be infected via several different means (routes). In all inoculated animals, specific anti-BLV antibodies can be demonstrated 1 to 3 months post-inoculation (p.i.). Between 10 and 13 months p.i., a moderate but persistent lymphocytosis (PL) may be detected in about 50% of the infected animals.
A L Parodi, Soufiane Djilali
openaire   +3 more sources

Breeding beef bulls as a source of BLV transmission

American Association of Bovine Practitioners Conference Proceedings, 2017
Bovine leukosis is a chronic lymphoproliferative disorder caused by bovine leukemia virus (BLV) that leads to economic losses in the beef and dairy industries. The USDA NAHMS Beef 1997 study estimated that 38% of cow-calf beef herds and 10.3% of individual adult cows in the US are BLV seropositive. Most BLV infected animals are asymptomatic carriers of
Daniel L. Grooms   +7 more
openaire   +2 more sources

The BLV-miRNAs pathway of BLV inhibits the expression of Lactoferrin, Lactoperoxidase, Alpha-lactalbumin and Beta-lactoglobulin proteins

Veterinary Microbiology
Bovine leukemia virus (BLV) is a widespread virus that decreases milk production and quality in dairy cows. As crucial components of BLV, BLV-encoded microRNAs (BLV-miRNAs) affect BLV replication and may impact the synthesis of Lactoferrin (LTF), Lactoperoxidase (LPO), Alpha-lactalbumin (alpha-LA), and Beta-lactoglobulin (beta-LG).
Shuai, Lian   +8 more
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In vitro accurate transcription from the cap site of bovine leukemia virus (BLV) dependent on the blv-infected cell nuclear lysate

Virology, 1992
The cell-free transcriptional system initiating from the cap site in bovine leukemia virus (BLV) LTR by RNA polymerase II was constructed. The transcription was completely dependent on the template DNA and the nuclear lysate isolated from BLV-infected bat lung cells (TB1Lu).
Akiko Shoji-Tanaka   +3 more
openaire   +3 more sources

Elicitation of bovine antibody to BLV-gp51 by BLV-vaccination.

Leukemia, 1989
This study was carried out to demonstrate sequential changes of antibody response to gp51 of BLV in bovine hosts injected with BLV-vaccine. BLV vaccine was prepared from culture fluids from FLK-BLV cells by treatment with 0.1% formalin for 48 hrs at 4 degrees C followed by ultrafiltration and lyophilization. Sterile vaccine containing 300 mg protein/ml,
K, Maruyama   +6 more
openaire   +1 more source

Biological activity of native BLV proteinase and C-terminal truncated BLV and HTLV-I proteinases

1994
Human T-cell leukemia virus is a T-cell lymphotropic retrovirus associated with T-cell leukemia and tropical spastic paraparesis, whereas bovine leukemia virus is the etiological agent of enzootic bovine lymphosarcoma. Bovine leukemia virus is reported as the animal model of human T-cell leukemia virus.
Précigoux, Gilles   +6 more
openaire   +1 more source

BLV proviral DNA in the genome of the target lymphocyte.

Archiv fur Geschwulstforschung, 1981
Integration of bovine leukemia proviral DNA in the genome of infected cells was investigated in cattle affected by either the persistent lymphocytosis or the lymph node tumor form of enzootic bovine leukosis. In persistent lymphocytosis, proviral DNA was found to be integrated at a large number of genomic sites in one-fourth to one-third of circulating
Kettmann, Richard   +3 more
openaire   +1 more source

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