Results 71 to 80 of about 4,208,103 (295)
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Introduction: Body temperature measurement in children is of clinical relevance. Although rectal measurement is the gold standard, less invasive tools have become available. We aimed to describe the accuracy of tympanic, infrared skin, or temporal artery
Karel Allegaert, MD, PhD +3 more
doaj +1 more source
Scan-rescan percent brain volume difference, number of failures, and scan-rescan percent brain volume difference.
Simon F. Eskildsen (5774273) +4 more
core +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima +8 more
wiley +1 more source
Scan Statistic and Multiple Scan Statistics
The scan statistic and multiple scan statistic can be used in many areas of science. In this chapter, a survey of results on scan statistic for the continuous conditional case is presented.
Lin, Chien-tai
core +1 more source
Discussion on the optimal acquisition and reconstruction protocol of uMI780 PET/CT by using 18 F-FDG
Objective: To investigate the optimal acquisition and reconstruction protocol of uMI780 positron emission tomography (PET)/computed tomography (CT) in the clinical application of 18 F-FDG for whole-body (head+body) imaging. Methods: A total of
SUN Zhen +12 more
doaj
Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley +1 more source
Hydrogel platforms for 3D cardiac tissue engineering and fibrotic disease modelling
Hydrogel‐based cardiac tissue models are advancing our understanding of heart health and disease. By incorporating electrical conductivity, aligned structures, and tunable mechanical properties, these platforms better replicate the complex environment of native heart tissue.
Harrison Porritt +2 more
wiley +1 more source
Power dissipation during scan testing is becoming an important concern as design sizes and gate densities increase. While several approaches have been recently proposed for reducing power dissipation during the shift cycle (minimum transition don't care ...
Rosinger, Paul +2 more
core +1 more source

