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Neutralizing antibodies to bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) and its subtypes

Veterinary Microbiology, 2010
This study was carried out to determine whether the sensitivity of serum neutralization (SN) tests would be affected by the use of distinct subtypes of bovine herpesvirus 1 (BoHV-1) and 5 (BoHV-5) as test challenge viruses. Bovine sera collected from a randomized sample (n=287) were tested in a 24h incubation SN against three type 1 viruses (BoHV-1.1 ...
A C Franco   +2 more
exaly   +3 more sources

A new method for simultaneous detection and discrimination of Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) using real time PCR with high resolution melting (HRM) analysis

Journal of Virological Methods, 2016
Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are antigenically and genetically similar. The aim of this study was to develop a simple and reliable one-step real time PCR assay with high resolution melting (HRM) analysis for the simultaneous detection and differentiation of BoHV-1 and BoHV-5. Optimization of assay conditions was performed with DNA
M S Marin   +2 more
exaly   +4 more sources

Recombinant BoHV-5 glycoprotein (rgD5) elicits long-lasting protective immunity in cattle

Virology, 2022
BoHV-5 is a worldwide distributed pathogen usually associated with a lethal neurological disease in dairy and beef cattle resulting in important economic losses due to the cattle industry. Using recombinant gD5, we evaluated the long-duration humoral immunity of the recombinant vaccines in a cattle model.
Itauá L. Araujo   +3 more
openaire   +2 more sources

Immunogenicity of an inactivated vaccine for intravaginal application against bovine alphaherpesvirus type 5 (BoHV-5)

Molecular Immunology, 2023
The present study was carried out to evaluate the intravaginal vaccine potential against bovine alphaherpesvirus type 5 (BoHV-5). Sixty three cows were divided into seven groups (n: 9) and inoculated intravaginally (VA) or intramuscularly (IM) with inactivated BoHV-5, associated with the recombinant B subunit of the heat-labile enterotoxin of E.
Cristina Mendes Peter   +10 more
openaire   +2 more sources

Bacillus Toyonensis BCT-7112T Spores as Parenteral Adjuvant of BoHV-5 Vaccine in a Murine Model

Probiotics and Antimicrobial Proteins, 2021
Bacterial spores of the genus Bacillus are being evaluated as adjuvant molecules capable of improving the immune response to vaccines. In this study, we investigate whether subcutaneously administered spores of B. toyonensis BCT-7112T could enhance a vaccine immune response in mice.
Francisco Denis Souza Santos   +6 more
openaire   +2 more sources

NTPDase and 5′-nucleotidase activities in synaptosomes of rabbits experimentally infected with BoHV-5

Journal of NeuroVirology, 2015
Bovine herpesvirus type 5 (BoHV-5) is the causative agent of herpetic meningoencephalitis in cattle. The purinergic system is described as a modulator of the immune response and neuroinflammation. These functions are related to the extracellular nucleotides concentration.
Cássia B, da Silva   +9 more
openaire   +2 more sources

Bovine Herpesvirus 5 (BoHV-5) in Bull Semen: Amplification and Sequence Analysis of the US4 Gene

Veterinary Research Communications, 2003
Bovine herpesvirus type 5 (BoHV-5), which is potentially neuropathogenic, was detected in clinical samples of bovine semen, both directly and after isolation in cell culture, using a nested PCR system for amplifying the US4 gene. Nucleotide sequences generated from the amplicons were analysed and deposited at GenBank (NCBI, Bethesda, MD, USA) under the
L I, Gomes   +4 more
openaire   +2 more sources

Role of the suppressor of cytokine signaling 2 (SOCS2) during meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5)

Comparative Immunology, Microbiology and Infectious Diseases, 2016
The role of suppressors of cytokine signaling (SOCS) in meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5) was evaluated by intracranial infection in C57BL/6 wild-type mice (WT) and SOCS2 deficient mice (SOCS2(-/-)). Both infected groups presented weight loss, ruffled fur and hunched posture.
Aline Aparecida Silva Barbosa   +16 more
openaire   +2 more sources

Cerebrospinal fluid changes in cattle with rabies or with BoHV-5 meningoencephalitis and its correlation with the severity of CNS inflammatory process

Research in Veterinary Science, 2018
Abstract The objective of this study was to describe and compare the changes in the cerebrospinal fluid (CSF) of cattle affected by rabies or BoHV-5 meningoencephalitis and correlating them with the severity of central nervous system (CNS) inflammation.
Gustavo Rodrigues Queiroz   +5 more
openaire   +2 more sources

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