Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow +12 more
wiley +1 more source
Facing migration under adverse conditions: challenges and resilience in the Colombo-Venezuelan border area. [PDF]
Bautista GO +6 more
europepmc +1 more source
Resurgence risk for malaria, and the characterization of a recent outbreak in an Amazonian border area between French Guiana and Brazil. [PDF]
Mosnier E +16 more
europepmc +1 more source
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Socioeconomic determinants of small and medium-sized dairy farms in the Ecuador-Colombia border area. [PDF]
Carvajal-Pérez LA +4 more
europepmc +1 more source
ATP measurement as an objective method to measure environmental contamination in 9 hospitals in the Dutch/Belgian border area. [PDF]
van Arkel A +16 more
europepmc +1 more source
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Transcultural Adaptation of the Breast Cancer Awareness Measure in a Rural Border Area of Greece. [PDF]
Papasozomenou P +5 more
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Molecular and serological investigations of Batai virus in cattle and goats in the border area of Yunnan, China (2021-2022). [PDF]
Lu Z +8 more
europepmc +1 more source

