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Transformation of Borrelia burgdorferi
Current Protocols, 2021AbstractTransformation techniques used to genetically manipulate Borrelia burgdorferi, the agent of Lyme disease, play a critical role in generating mutants that facilitate analyses of the role of genes in the pathophysiology of this bacterium.
Janakiram Seshu +2 more
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Borrelia burgdorferi and localized scleroderma
Clinics in Dermatology, 1994L yme borreliosis is a multisystemic disorder caused by the spirochete Borrelia burgdorferi and transmitted by the Ixodes tick. The initial manifestations of the disease are the characteristic skin lesion, erythema migrans, and symptoms of general malaise (stage I).
TREVISAN, GIUSTO +2 more
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Detection of glycoproteins in Borrelia burgdorferi
Archives of Microbiology, 1992The presence of carbohydrates on proteins of Borrelia burgdorferi, the causative agent of Lyme disease, was investigated by using a digoxigenin labeling method together with Schiff staining and N-glycosidase F assay. The two major outer surface exposed proteins of 31 kDa and 34 kDa showed to be glycosylated and gel filtration high pressure liquid ...
Roberto Cevenini +2 more
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Borrelia burgdorferi Microarray Analysis [PDF]
, 2017 Microarray studies have contributed significantly to the current understanding of Borrelia burgdorferi genome content and transcriptional regulation. Here, we describe the use of microarray technology for several aspects of B. burgdorferi genomic analysis.
Radha Iyer, Ira Schwartz
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Microbiology of Borrelia burgdorferi
Seminars in Neurology, 1997This article reviews the natural history, taxonomy, physical structure, growth requirements, and molecular structure of Borrelia burgdorferi sensu lato, the causative agent of Lyme disease. These spirochetal bacteria are maintained in nature through an infectious cycle between wild mammals and ticks.
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Asymptomatic Borrelia burgdorferi infection
Medical Hypotheses, 2001Little is known about the natural history of asymptomatic Borrelia burgdorferi infection. Our analysis of the asymptomatic infections diagnosed serologically in a recent OspA vaccine trial conducted in the United States (N Engl J Med 1998;339: 209-215), suggests that the natural history of this event is more benign than that reported for untreated ...
Ira Schwartz +3 more
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2002
Publisher Summary Borrelia burgdorferi causes Lyme borreliosis (LB) or Lyme disease. It possesses some genetic and phenotypic features that are unique among prokaryotes, such as a linear chromosome and multiple linear and circular plasmids in a single cell. B.
Guiqing Wang +2 more
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Publisher Summary Borrelia burgdorferi causes Lyme borreliosis (LB) or Lyme disease. It possesses some genetic and phenotypic features that are unique among prokaryotes, such as a linear chromosome and multiple linear and circular plasmids in a single cell. B.
Guiqing Wang +2 more
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Nonprotein Antigens of Borrelia burgdorferi
Journal of Infectious Diseases, 1993Preparative thin-layer chromatograms of chloroform-methanol extracts of Borrelia burgdorferi (B31) sonicates showed four fractions (Rf values of 0.84, 0.81, 0.66 and 0.61) that stained with iodine vapors, orcinol, or phospray, suggesting the presence of lipid-, carbohydrate-, and phosphorus-containing compounds.
J. C. Garcia Monco +5 more
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The recA gene of Borrelia burgdorferi
Gene, 1995The nucleotide sequence of the Borrelia burgdorferi (Bb) Sh-2-82 recA gene has been determined using PCR-based approaches without the construction of a genomic library. The gene should encode a protein of 365 amino acids which is highly homologous to other known RecA proteins.
Wai Mun Huang, Dew-Jager K, Weng-Qing Yu
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