Ultra-high resolution X-ray structure of orthorhombic bovine pancreatic Ribonuclease A at 100K [PDF]
The crystal structure of orthorhombic Bovine Pancreatic Ribonuclease A has been determined to 0.85 Å resolution using low temperature, 100 K, synchrotron X-ray data collected at 16000 keV (λ = 0.77 Å). This is the first ultra-high-resolution structure of
David R. Lisgarten +9 more
doaj +2 more sources
Enhancement of the activity of the antimicrobial peptides HNP1 and LL-37 by bovine pancreatic ribonuclease A [version 3; peer review: 1 approved, 2 approved with reservations, 1 not approved] [PDF]
Background: HNP1, LL-37, and HBD1 are antimicrobial against Escherichia coli ATCC 25922 at the standard inoculum but less active at higher inocula. Methods: The virtual colony count (VCC) microbiological assay was adapted for high inocula and the ...
Bryan Ericksen
doaj +2 more sources
Conformational Studies on a Glycosylated Bovine Pancreatic Ribonuclease
Abstract Various spectral properties (circular dichroism, optical rotatory dispersion, and ultraviolet denaturation difference spectroscopy) of a glycosylated bovine pancreatic ribonuclease (RNase B') have been measured. This glycoprotein contains an average of 1 mole of glucosamine and 2 to 3 moles of mannose per mole of protein.
David Puett
openaire +3 more sources
Bovine pancreatic ribonuclease: fifty years of the first enzymatic reaction mechanism. [PDF]
Cuchillo CM, Nogués MV, Raines RT.
europepmc +3 more sources
Expression of bovine pancreatic ribonuclease A in Escherichia coli [PDF]
A synthetic gene for bovine pancreatic ribonuclease A (RNase A) has been expressed in Escherichia coli as a fusion protein with β‐galactosidase linked by the tetrapeptide Ile‐Glu‐Gly‐Arg. RNase A was cleaved from the fusion using factor Xa, and the resulting product prufied and reconstituted. The isolated RNase A was chromatographically, catalytically,
K P, Nambiar +3 more
openaire +3 more sources
Kinetic and thermodynamic analysis of the conformational folding process of SS-reduced bovine pancreatic ribonuclease A using a selenoxide reagent with high oxidizing ability. [PDF]
Arai K, Kumakura F, Iwaoka M.
europepmc +3 more sources
Molecular dynamics simulation of bovine pancreatic ribonuclease A-CpA and transition state-like complexes. [PDF]
Formoso E, Matxain JM, Lopez X, York DM.
europepmc +3 more sources
On the Preparation of Bovine Pancreatic Ribonuclease A
A M, CRESTFIELD, W H, STEIN, S, MOORE
openaire +3 more sources
Production of ovine Pancreatic Ribonuclease and investigation of enzyme characteristics [PDF]
Introduction Obviously, the recent decades strategy in cancer therapy, anticancer drug discovery and drug improvement is to characterize, distinguish and validate the most promising cancer-related molecular targets to which new drugs can be designed. The
mahsa Zabetian +3 more
doaj +1 more source
Ribonucleases as potential therapeutic agents [PDF]
Objective Due to high cytotoxicity effect, ribonucleases (RNases) are known as pharmacological agents with therapeutic potential. The high potential of these enzymes in the destruction of RNA strand and their other substrates and subsequently, cell ...
Masoume Vakili-Azghandi +3 more
doaj +1 more source

