Results 1 to 10 of about 14,129 (184)

Ultra-high resolution X-ray structure of orthorhombic bovine pancreatic Ribonuclease A at 100K [PDF]

BMC Chemistry, 2023
The crystal structure of orthorhombic Bovine Pancreatic Ribonuclease A has been determined to 0.85 Å resolution using low temperature, 100 K, synchrotron X-ray data collected at 16000 keV (λ = 0.77 Å). This is the first ultra-high-resolution structure of
David R. Lisgarten, Rex A. Palmer, Jon B. Cooper, Claire E. Naylor, Rosemary C. Talbert, Brendan J. Howlin, John N. Lisgarten, Janez Konc, Shabir Najmudin, Carina M. C. Lobley   +9 more
doaj   +2 more sources

Enhancement of the activity of the antimicrobial peptides HNP1 and LL-37 by bovine pancreatic ribonuclease A [version 3; peer review: 1 approved, 2 approved with reservations, 1 not approved] [PDF]

F1000Research, 2023
Background: HNP1, LL-37, and HBD1 are antimicrobial against Escherichia coli ATCC 25922 at the standard inoculum but less active at higher inocula.   Methods: The virtual colony count (VCC) microbiological assay was adapted for high inocula and the ...
Bryan Ericksen
doaj   +2 more sources

Production of ovine Pancreatic Ribonuclease and investigation of enzyme characteristics [PDF]

پژوهشهای علوم دامی ایران, 2021
Introduction Obviously, the recent decades strategy in cancer therapy, anticancer drug discovery and drug improvement is to characterize, distinguish and validate the most promising cancer-related molecular targets to which new drugs can be designed. The
mahsa Zabetian, Mohammad reza Nasiri, Ali Javadmannesh, Shahrokh ghovati   +3 more
doaj   +1 more source

Ribonucleases as potential therapeutic agents [PDF]

مجله بیوتکنولوژی کشاورزی, 2021
Objective Due to high cytotoxicity effect, ribonucleases (RNases) are known as pharmacological agents with therapeutic potential. The high potential of these enzymes in the destruction of RNA strand and their other substrates and subsequently, cell ...
Masoume Vakili-Azghandi, Mohammadreza Nasiri, Shahrokh Ghovvati, Ali Javadmanesh   +3 more
doaj   +1 more source

Engineering and production of recombinant bovine pancreatic ribonuclease enzyme (RNase A) as a potential therapeutic [PDF]

مجله بیوتکنولوژی کشاورزی, 2021
Objective  Immunotoxins are one of the most promising ways in therapeutic fields, specially cancer therapy which have a unique toxin-antibody structure, and kill the cancer cell by passing through the cell membrane and entering the target cell.
Masoume Vakili-Azghandi, Mohammadreza Nassiri, Shahrokh Ghovvati, Ali Javadmanesh   +3 more
doaj   +1 more source

PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells [PDF]

BioImpacts, 2020
Introduction: Currently, drug-induced reactive oxygen species (ROS) mediating apoptosis pathway have extensively been investigated in designing effective strategies for colorectal cancer (CRC) chemotherapy.
Mostafa Akbarzadeh Khiavi, Azam Safary, Jaleh Barar, Hamed Farzi-Khajeh, Abolfazl Barzegari, Rahimeh Mousavi, Mohammad Hossein Somi, Yadollah Omidi   +7 more
doaj   +1 more source

Bovine Pancreatic RNase A: An Insight into the Mechanism of Antitumor Activity In Vitro and In Vivo

Pharmaceutics, 2022
In this investigation, we extensively studied the mechanism of antitumor activity of bovine pancreatic RNase A. Using confocal microscopy, we show that after RNase A penetration into HeLa and B16 cells, a part of the enzyme remains unbound with the ...
Islam Saber Ead Mohamed, Aleksandra V. Sen’kova, Oleg V. Markov, Andrey V. Markov, Innokenty A. Savin, Marina A. Zenkova, Nadezhda L. Mironova   +6 more
doaj   +1 more source

Revisiting the Formation of a Native Disulfide Bond: Consequences for Protein Regeneration and Beyond

Molecules, 2020
Oxidative protein folding involves the formation of disulfide bonds and the regeneration of native structure (N) from the fully reduced and unfolded protein (R).
Mahesh Narayan
doaj   +1 more source

NMR studies on structure and dynamics of the monomeric derivative of BS-RNase: new insights for 3D domain swapping. [PDF]

PLoS ONE, 2012
Three-dimensional domain swapping is a common phenomenon in pancreatic-like ribonucleases. In the aggregated state, these proteins acquire new biological functions, including selective cytotoxicity against tumour cells.
Roberta Spadaccini, Carmine Ercole, Maria A Gentile, Domenico Sanfelice, Rolf Boelens, Rainer Wechselberger, Gyula Batta, Andrea Bernini, Neri Niccolai, Delia Picone   +9 more
doaj   +1 more source

Protein disulfide-isomerase interacts with a substrate protein at all stages along its folding pathway [PDF]

, 2014
In contrast to molecular chaperones that couple protein folding to ATP hydrolysis, protein disulfide-isomerase (PDI) catalyzes protein folding coupled to formation of disulfide bonds (oxidative folding).
A Jansens, A Land, A Pirneskoski, A. Katrine Wallis, Alistair G. Irvine, AR Karala, AR Karala, AY Denisov, B van den Berg, B van den Berg, B van den Berg, CB Anfinsen, Claudia A. Blindauer, CPM van Mierlo, CPM van Mierlo, CW Gruber, DP Goldenberg, E van Anken, F Hatahet, G Kozlov, G Wagner, HC Shin, HC Shin, J Riemer, JH Laity, JL Arolas, JS Weissman, JS Weissman, L Wang, LJ Byrne, Lloyd W. Ruddock, M Narayan, Mark J. Howard, Michelle L. Rowe, Narinder Sanghera, NJ Bulleid, NJ Darby, P Klappa, P Venetianer, RA Williamson, RB Freedman, RF Goldberger, Richard A. Williamson, RJ Ellis, Robert B. Freedman, S Masui, S Talluri, Salvador Ventura, TE Creighton, TE Creighton, TE Creighton, TE Creighton, VD Nguyen, VD Nguyen, WF Vranken   +54 more
core   +3 more sources