Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Comparison of clinical outcomes between plastic braces versus sponge abduction packs in the early rehabilitation period after rotator cuff repair. [PDF]
Du R +6 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Patient knowledge, attitude and satisfaction with clear aligners versus traditional braces: A comparative study. [PDF]
Sharma S +5 more
europepmc +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Comparative analysis of artificial intelligence chatbots in orthodontic emergency scenarios: ChatGPT-3.5, ChatGPT-4.0, Copilot, and Gemini. [PDF]
Erdem B, Özcan M, Şar Ç.
europepmc +1 more source
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Advertising of orthodontic appliances on websites in the UK: Do they comply with advertising standards? A cross-sectional study. [PDF]
Nehra A, Jones A, Hodge T.
europepmc +1 more source
Classification of temporomandibular joint hypermobility based on lateral TMJ, and magnetic resonance imaging contributing to a nonsurgical treatment protocol. [PDF]
Hegab AF +3 more
europepmc +1 more source
Comparative Effectiveness of Combined Orthotic and Physiotherapy Interventions Versus Monotherapy in the Management of Knee Osteoarthritis on Clinical Parameters: A Systematic Review of Randomized Controlled Trials. [PDF]
Zangi M +5 more
europepmc +1 more source

