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Hepatic Histopathological Benefit, Microbial Cost: Oral Vancomycin Mitigates Non-Alcoholic Fatty Liver Disease While Disrupting the Cecal Microbiota. [PDF]
Int J Mol SciÇirkin G +8 more
europepmc +1 more source
PCR detection ofBrachyspira aalborgiandBrachyspira pilosicoliin human faeces [PDF]
FEMS Microbiology Letters, 2001 Previously-developed PCR protocols specific for the 16S rRNA gene of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli were adapted for the detection of these species in human faeces, following DNA extraction and purification using mini-prep columns. The limits of detection in seeded faeces for B. aalborgi and B.
David J Hampson
exaly +4 more sources
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Rapid isolation of Brachyspira hyodysenteriae and Brachyspira pilosicoli from pigs
Veterinary Microbiology, 2005The aim of this study was to compare and evaluate the time required to isolate Brachyspira hyodysenteriae and Brachyspira pilosicoli from porcine faeces. This was done using previously described selective media (spectinomycin) S400, (colistin, vancomycin and spectinomycin) CVS and (spectinomycin, vancomycin, colistin, spiramycin and rifampin with swine
Adriana Calderaro
exaly +4 more sources
Brachyspira intermedia and Brachyspira pilosicoli Are Commonly Found in Older Laying Flocks in Pennsylvania [PDF]
Avian Diseases, 2009 Anaerobic intestinal spirochetes (genus Brachyspira) include several species that are recognized as pathogens of poultry. Surveys undertaken in Europe and Australia have shown that layer and breeder flocks are often colonized by the pathogenic species Brachyspira intermedia and Brachyspira pilosicoli, but similar surveys have not been conducted in the ...
David J Hampson
exaly +3 more sources
Veterinary Microbiology, 2010
The aims of this study were to investigate the prevalence of colonization with intestinal spirochaetes in dogs, and to assess their association with diarrhoea. To achieve this, faecal samples from 311 dogs were obtained between November 2008 and April 2009 and cultured for Brachyspira species. A total of 41 Brachyspira spp. isolates were recovered, and
Alvaro, Hidalgo +3 more
openaire +2 more sources
The aims of this study were to investigate the prevalence of colonization with intestinal spirochaetes in dogs, and to assess their association with diarrhoea. To achieve this, faecal samples from 311 dogs were obtained between November 2008 and April 2009 and cultured for Brachyspira species. A total of 41 Brachyspira spp. isolates were recovered, and
Alvaro, Hidalgo +3 more
openaire +2 more sources
An update of Brachyspira hyodysenteriae serotyping
Research in Veterinary Science, 2017Brachyspira (B.) hyodysenteriae the causative agent of swine dysentery (SD) has been divided into 9 serotypes on basis of its lipooligosaccharide (LOS). Knowledge on circulating serotypes in Europe, however, is rare. Regarding that immunity to SD is serotype specific an update of B. hyodysenteriae serotyping was undertaken.
Werner, Herbst +3 more
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Journal of Microbiological Methods, 2006
Traditional culture and biochemical tests (CBT) were compared with PCR for sensitivity and detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in seeded faeces and clinical samples from diarrhoeic pigs. A duplex PCR system was developed based on primers detecting the tlyA-gene of B. hyodysenteriae and the 16S rRNA-gene of B.
T, Råsbäck +3 more
openaire +2 more sources
Traditional culture and biochemical tests (CBT) were compared with PCR for sensitivity and detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in seeded faeces and clinical samples from diarrhoeic pigs. A duplex PCR system was developed based on primers detecting the tlyA-gene of B. hyodysenteriae and the 16S rRNA-gene of B.
T, Råsbäck +3 more
openaire +2 more sources
Journal of Medical Microbiology, 2004
Porcine intestinal spirochaetes are fastidious anaerobic organisms and, as a consequence, it has been necessary to develop various protocols to enhance their isolation from or detection in faeces. Immunomagnetic separation (IMS) is a method developed recently to improve separation of target cells from mixed cell suspensions.
Corona-Barrera, E. +4 more
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Porcine intestinal spirochaetes are fastidious anaerobic organisms and, as a consequence, it has been necessary to develop various protocols to enhance their isolation from or detection in faeces. Immunomagnetic separation (IMS) is a method developed recently to improve separation of target cells from mixed cell suspensions.
Corona-Barrera, E. +4 more
openaire +2 more sources
Brachyspira murdochii Colitis in Pigs
Veterinary Pathology, 2009The weakly beta-hemolytic porcine spirochete Brachyspira murdochii is considered a normal intestinal commensal. In the present study, however, a field case of B murdochii–associated catarrhal colitis was identified in a pig, as characterized by extensive spirochetal colonization of the surface epithelium.
T K, Jensen, A S, Christensen, M, Boye
openaire +2 more sources