Results 111 to 120 of about 547 (139)

PCR detection ofBrachyspira aalborgiandBrachyspira pilosicoliin human faeces [PDF]

open access: yesFEMS Microbiology Letters, 2001
Previously-developed PCR protocols specific for the 16S rRNA gene of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli were adapted for the detection of these species in human faeces, following DNA extraction and purification using mini-prep columns. The limits of detection in seeded faeces for B. aalborgi and B.
David Hampson, A S Mikosza, T La
exaly   +4 more sources

Brachyspira pilosicoli Isolated from Pigs in Japan

open access: yesJournal of Veterinary Medical Science, 2004
Two of four weak beta-hemolytic isolates of intestinal spirochetes isolated from pigs in Japan possessed a unique base alignment of TTTTTT on the 16S ribosomal DNA of Brachyspira pilosicoli and were identified as B. pilosicoli. The other two isolates were not identified by this technique.
Yoshikazu Adachi
exaly   +4 more sources

Rapid isolation of Brachyspira hyodysenteriae and Brachyspira pilosicoli from pigs

Veterinary Microbiology, 2005
The aim of this study was to compare and evaluate the time required to isolate Brachyspira hyodysenteriae and Brachyspira pilosicoli from porcine faeces. This was done using previously described selective media (spectinomycin) S400, (colistin, vancomycin and spectinomycin) CVS and (spectinomycin, vancomycin, colistin, spiramycin and rifampin with swine
A Calderaro, C Chezzi, G Dettori
exaly   +4 more sources

Brachyspira (Serpulina) pilosicoli Spirochetemia in an Immunocompromised Patient

Infection, 2002
The case of an elderly immunocompromised man with non-Hodgkin's lymphoma who presented with fever, abdominal pain and bloody diarrhea is described. Brachyspira pilosicoli was isolated from culture. The patient was treated with penicillin G i.v. and became afebrile. B.
E Mantadakis
exaly   +3 more sources

Prevalence of Brachyspira pilosicoli and “Brachyspira canis” in dogs and their association with diarrhoea

Veterinary Microbiology, 2010
The aims of this study were to investigate the prevalence of colonization with intestinal spirochaetes in dogs, and to assess their association with diarrhoea. To achieve this, faecal samples from 311 dogs were obtained between November 2008 and April 2009 and cultured for Brachyspira species. A total of 41 Brachyspira spp. isolates were recovered, and
Alvaro, Hidalgo   +3 more
openaire   +2 more sources

Tiamulin resistance in porcine Brachyspira pilosicoli isolates

Research in Veterinary Science, 2006
There are few studies on antimicrobial susceptibility of Brachyspira pilosicoli, therefore this study was performed to investigate the situation among isolates from pigs. The tiamulin and tylosin susceptibility was determined by broth dilution for 93 and 86 porcine B. pilosicoli isolates, respectively.
M, Pringle, A, Landén, A, Franklin
openaire   +2 more sources

Colonisation of pet shop puppies with Brachyspira pilosicoli

Veterinary Microbiology, 2003
Anaerobic intestinal spirochaetes of the genus Brachyspira are known to colonise dogs, but relatively little is known about their prevalence, distribution or pathogenic potential. One species, Brachyspira pilosicoli, is thought to cause diarrhoea in dogs, as well as in other animals and humans.
Oxberry, S.L., Hampson, D.J.
openaire   +2 more sources

Comparison of culture and biochemical tests with PCR for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli

Journal of Microbiological Methods, 2006
Traditional culture and biochemical tests (CBT) were compared with PCR for sensitivity and detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in seeded faeces and clinical samples from diarrhoeic pigs. A duplex PCR system was developed based on primers detecting the tlyA-gene of B. hyodysenteriae and the 16S rRNA-gene of B.
T, Råsbäck   +3 more
openaire   +2 more sources

Extensive intestinal spirochaetosis in pigs challenged with Brachyspira pilosicoli

Journal of Medical Microbiology, 2004
A field isolate of Brachyspira pilosicoli, the aetiological agent of porcine intestinal spirochaetosis, was inoculated by stomach tube into six 6-week-old pigs. All animals developed loose to watery faeces and were killed successively on days 8, 14 or 17 post-inoculation (day 17 being the end of the study).
Tim K, Jensen   +2 more
openaire   +2 more sources

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