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Electroporation of Bradyrhizobium japonicum
Molecular and General Genetics MGG, 1990Electroporation offers a fast, efficient and reproducible way to introduce DNA into bacteria. We have successfully used this technique to transform two commercially important strains of Bradyrhizobium japonicum, the nitrogen-fixing soybean symbiont. Initially, electroporation conditions were optimized using plasmid DNA which had been prepared from the ...
Mary Lou Guerinot +2 more
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Viability of Bradyrhizobium japonicum bacteriods
Archives of Microbiology, 1987Homogenates from soybean nodules, formed by 12 strains of Bradyrhizobium japonicum, were plated into yeast-extract mannitol agar containing 3 or 37 g mannitol 1-1. Viable counts ranged from 8.298 to 11.265 log10 cells-gram nodule-1. When monitored over the life cycle of the symbiosis, the viability of strains USDA 110 and USDA 123 increased with days ...
T. R. McDermott +2 more
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2011
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BRADYRHIZOBIUM JAPONICUM AND PHOSPHOBACTERIA FOR SOYBEAN
Madras Agricultural Journal, 1998The effect of individual and combined inoculations of Bradyrhizobiumand phosphobacteria along with application of super phosphate. rock phosphate and FYM on the growth and yield of soybean were studied. The combined inoculation of Bradyrhizobium and phosphobacteria was proved to be better than individual inoculations. The combined inoculations with FYM+
SHEERIN S +2 more
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Degradation of catechin by Bradyrhizobium japonicum
Biodegradation, 1997Rhizobia utilize phenolic substances as sole carbonsource. Bradyrhizobium japonicum utilizescatechin, a unit of condensed tannin as carbonsource. To establish the degradative pathway ofcatechin, the products of catechin degradation wereisolated by paper chromatography and TLC andidentified by HPLC, UV, IR and NMR spectra.
Waheeta Hopper, A. Mahadevan
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2009
Influence of the inoculation of soybean by new Tn5-mutants of Bradyrhizobium japonicum 646 on seed productivity and change of nitrogen fixing activity (NFA) and photosynthetic rate (PR) on the different stages of plant development were studied in vegetative experiment.
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Influence of the inoculation of soybean by new Tn5-mutants of Bradyrhizobium japonicum 646 on seed productivity and change of nitrogen fixing activity (NFA) and photosynthetic rate (PR) on the different stages of plant development were studied in vegetative experiment.
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Repeated sequence RSa is diagnostic for Bradyrhizobium japonicum and Bradyrhizobium elkanii
Biology and Fertility of Soils, 1996The genome of Bradyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B.
Hartmann, A. +3 more
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A Motility Revertant of the ndvB Mutant of Bradyrhizobium japonicum
Current Microbiology, 2003A motility revertant of a Bradyrhizobium japonicum ndvB mutant was isolated and characterized. The ndvB mutants of B. japonicum have been reported to be osmotically sensitive, as well as defective in motility, periplasmic cyclic beta-(1-->3), (1-->6)-D-glucan synthesis, and symbiosis with soybean.
Rongji, Chen +2 more
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2014
As a result of screening Tn5-mutants of Bradyrhizobium japonicum, which were obtained using vector pSUP5051::Tn5mob on the characteristics of ??virulence??, ??nitrogen fixation??, ??efficiency of symbiosis??, genetically marked rhizobia B-2, B-16, B-18, B-20, B-21 with improved properties were selected.
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As a result of screening Tn5-mutants of Bradyrhizobium japonicum, which were obtained using vector pSUP5051::Tn5mob on the characteristics of ??virulence??, ??nitrogen fixation??, ??efficiency of symbiosis??, genetically marked rhizobia B-2, B-16, B-18, B-20, B-21 with improved properties were selected.
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2015
The influence of different ways of exogenous soybean seed lectin application on the intensity of photosynthesis and the grain productivity of soybean is investigated. The close correlation of the grain productivity of plants with the intensity of photosynthesis and nitrogen fixation is found.
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The influence of different ways of exogenous soybean seed lectin application on the intensity of photosynthesis and the grain productivity of soybean is investigated. The close correlation of the grain productivity of plants with the intensity of photosynthesis and nitrogen fixation is found.
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