Results 301 to 310 of about 298,071 (357)

Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations

Biotechnology Progress, EarlyView.
Abstract Phocaeicola vulgatus (formerly Bacteroides vulgatus), an anaerobic gut bacterium, produces several organic acids. Research on P. vulgatus is still in its infancy. However, a detailed understanding of P. vulgatus growth and metabolism is essential for its assessment as an organic acid producer.
Laura Keitel, Benjamin Schick, Gino Pohen, Stanislav Yordanov, Jochen Büchs   +4 more
wiley   +1 more source

Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell concentrations

Biotechnology Progress, EarlyView.
Abstract The emergence of new viruses and the spread of existing pathogens necessitate efficient vaccine production methods. The baculovirus expression vector system (BEVS) is an efficient and scalable system for subunit and virus‐like particle vaccine production and gene therapy vectors.
Jort J. Altenburg, Brenda E. Juarez‐Garza, Jelle van Keimpema, Linda van Oosten, Gorben P. Pijlman, Monique M. van Oers, René H. Wijffels, Dirk E. Martens   +7 more
wiley   +1 more source

Improving outcomes in intensified processing via optimization of the cell line development workflow

Biotechnology Progress, EarlyView.
Abstract As the industry continues to explore the benefits of continuous and intensified manufacturing, it is important to assure that the cell line development (CLD) workflows in practice today are well suited to generate clones that meet the unique challenges associated with these processes. Most cell lines used in intensified processes are currently
Vincent Balassi, Mary Otto, Corey Kretzmer, Amber Petersen, Channing McLaurin, Jana Mahadevan, Jason Gustin, Trissa Borgschulte, David Razafsky   +8 more
wiley   +1 more source

Iterative hybrid model based optimization of rAAV production

Biotechnology Progress, EarlyView.
Abstract Changes in serotype or genetic payload of recombinant adeno associated virus (rAAVs) gene therapies require adapting the transfection conditions of the upstream HEK293 cultivations. This study adopts an iterative model‐based experiment design approach, where increasing data availability is leveraged to evolve models of different complexity ...
Claudio Müller, Gerald Siegwart, Susanne Heider, Michael Sokolov, Angela Botros, Alexandra Umprecht, Moritz von Stosch, Mariano Nicolas Cruz Bournazou   +7 more
wiley   +1 more source

Breast-feeding during colchicine therapy for familial Mediterranean fever

, 1991
Jeffrey M. Milunsky, Aubrey Milunsky
openalex   +1 more source

N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors

Biotechnology Progress, EarlyView.
Abstract One strategy to enhance the production of biological therapeutics is using transient perfusion in the preculture (N‐1 stage) to seed the production culture (N stage) at ultra‐high cell densities (>10 x 106 viable cells/mL). This very high seeding density improves cell culture performance by shortening the timeline and/or achieving higher final
Lucas Lemire, Sebastian‐Juan Reyes, Yves Durocher, Robert Voyer, Olivier Henry, Phuong Lan Pham   +5 more
wiley   +1 more source

Needs and contents of a customized digital tool for retention in care and medication adherence among pregnant and breast-feeding women living with HIV in Kilimanjaro

, 2023
Maro RA, Ngowi K, Martin H, Dillip A, Masika L, Mtenga A, Mtesha B, Boer MS.   +7 more
europepmc   +1 more source

27 MODULATION OF IGE AND IGG RESPONSES BY FEEDING “HYPOALLERGENIC” OR ADAPTED FORMULAE BEFORE BREAST FEEDING IN NORMAL INFANTS

, 1992
S. Strohel, B. Digeon, Claude Chastang, E. Gibert, D Dupouy, Lise Leroux, M Robillard, M O Gailing, J. Schmitz Hôp, Estelle Paris   +9 more
openalex   +1 more source

Continuous purification of a parvovirus using two aqueous two‐phase extraction steps

Biotechnology Progress, EarlyView.
Abstract Aqueous two‐phase systems (ATPS) are a liquid–liquid extraction method that offers low‐cost, continuous‐adaptable virus purification. A two‐step ATPS using polyethylene glycol (PEG) and sodium citrate that recovered 66% of infectious porcine parvovirus with 2.0 logs of protein removal and 1.0 logs of DNA removal in batch has now been run ...
Natalie M. Nold, Sheridan Waldack, Grace James, Trisha Colling, Lynn Manchester, Taravat Sarvari, Amanda Bekkala, Seth A. Kriz, Madison Baldwin, Emily Agustin‐Mazariegos, Michael J. Betenbaugh, Caryn L. Heldt   +11 more
wiley   +1 more source