Results 161 to 170 of about 274,477 (261)

PAIR: Reconstructing Single‐Cell Open‐Chromatin Landscapes for Transcription Factor Regulome Mapping

open access: yesAdvanced Science, EarlyView.
scATAC‐seq analysis is often constrained by limited sequencing depth, extreme sparsity, and pervasive technical missingness. PAIR is a probabilistic framework that restores scATAC‐seq accessibility profiles by directly modeling the native cell–peak bipartite structure of chromatin accessibility.
Yanchi Su   +7 more
wiley   +1 more source

From Cell‐Free Transcriptomes to Single‐Cell Landscapes: Biomarker Discovery and Originating Cell Alteration Analysis via Graph Matrix Factorization

open access: yesAdvanced Science, EarlyView.
CellFreeGMF traces plasma cfRNA to likely originating cell types by integrating single‐cell atlases with graph‐regularized matrix factorization. The method decomposes cfRNA profiles into sample–cell contributions to reconstruct pseudo single‐cell expression.
Wenxiang Zhang   +9 more
wiley   +1 more source

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

open access: yesAdvanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

Bioinspired Morphology‐Decoupled Soft Gripper with Enhanced Bidirectional Grasping Capability

open access: yesAdvanced Science, EarlyView.
Inspired by biological predation, a novel gripper decouples cross‐modal grasping via dual morphological configurations. Synergistically integrating hybrid rigid and soft coupled fingers with a metamaterial palm, the system performs active compliant grasping for static objects and passive cage capture for dynamic targets.
Yedong Huang   +4 more
wiley   +1 more source

Cotton Recruits Soil‐Derived Delftia tsuruhatensis to Suppress Aphid Detoxification Via Salicylic Acid‐Mediated Defense

open access: yesAdvanced Science, EarlyView.
This study systematically reveals a complex interactive network involving plants, microbes, and insects, elucidating the ecological and molecular mechanisms by which cotton enhances its resistance to aphids through the active recruitment of the beneficial soil bacterium Delftia tsuruhatensis.
Hui Xue   +11 more
wiley   +1 more source

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