Results 41 to 50 of about 11,369 (183)

Two‐Dimensional Materials For Lateral Flow Assays: A Critical Review of Functional Roles and Translational Challenges

VIEW, EarlyView.
2D nanosheets, with large surface area, excellent optical/electrical properties, and high specificity, combine with lateral flow assay strips to enable rapid, readable detection, boosting the strips’ development and applications. Abstract Lateral flow assays (LFAs) are widely used for point‐of‐care diagnostics, but their detection capability remains ...
Weimin Xu, Ruping Liu, Na Gao, Jianxiao Zhu, Haowen Jiang, Shouzheng Jiao, Zhicheng Sun, Kai Shen, Gaolian Xu, Minghua Du, Ziyan Li, Yang Wang   +11 more
wiley   +1 more source

Mitochondrial fusion protein 2 affects intracellular survival of brucella abortus a19 by regulating endoplasmic reticulum stress and apoptosis [PDF]

Kafkas Universitesi Veteriner Fakültesi Dergisi
Mitochondrial fusion protein 2 (MFN2) deficiency has been shown to hinder the survival of bacteria in macrophages. Endoplasmic reticulum stress and apoptosis are vital defense mechanisms against Brucella infection, but the specific role of MFN2 in ...
Zhenyu XU, Qin YANG, Xiaoyu DENG, Yimei XU, Zhongchen MA, Chuangfu CHEN   +5 more
doaj   +1 more source

Specific Detection and Differentiation Between Brucella melitensis and Brucella abortus by a Duplex Recombinase Polymerase Amplification Assay

Frontiers in Veterinary Science, 2020
Brucellosis is a highly contagious zoonosis caused by a species under the genus Brucella. A duplex recombinase polymerase amplification (Duplex RPA) assay for the specific detection of Brucella melitensis and Brucella abortus was developed in this study.
M. M. Gumaa, M. M. Gumaa, Zhaocai Li, Xiaoan Cao, Nianzhang Zhang, Zhongzi Lou, Jizhang Zhou, Baoquan Fu   +7 more
doaj   +1 more source

Antibiotic persistence of intracellular Brucella abortus

PLOS Neglected Tropical Diseases, 2022
Background Human brucellosis caused by the facultative intracellular pathogen Brucella spp. is an endemic bacterial zoonosis manifesting as acute or chronic infections with high morbidity. Treatment typically involves a combination therapy of two antibiotics for several weeks to months, but despite this harsh treatment relapses occur at a rate of 5–15%
Selma Mode, Maren Ketterer, Maxime Québatte, Christoph Dehio   +3 more
openaire   +4 more sources

Toward understanding the catalytic mechanism for two classes of bacterial phospholipid N‐methyltransferases

The FEBS Journal, EarlyView.
Phospholipid N‐methyltransferases (Pmts) synthesize phosphatidylcholine in many bacteria. In this study, we compared the catalytic mechanisms of two bacterial Pmt classes: the Rhodobacter (R‐) and the Sinorhizobium (S‐) type. Representative enzymes for each class were derived from Rubellimicrobium thermophilum and Agrobacterium tumefaciens ...
Irina Shevyreva, Lena Sophie Fritsch, Leander Mika Koch, Anton Effing, Meriyem Aktas, Franz Narberhaus   +5 more
wiley   +1 more source

Effects of live Brucella abortus strain 19 vaccine on reindeer

Rangifer, 1989
Twenty female and seven male reindeer (Rangifer tarandus) were vaccinated subcutaneously in the right shoulder with a 1-ml dose of approximately 1.2 x 108 colony forming units of Brucella abortus strain 19, the standard reduced dose for cattle.
Robert A. Dieterich, Jamie K. Morton
doaj   +1 more source

Structural and thermodynamic analyses of a novel β‐1,2‐glucan binding mode in the ABC transporter solute‐binding protein Chy400_4166 from Chloroflexus aurantiacus

The FEBS Journal, EarlyView.
Cyclic β‐1,2‐glucan (CβG)‐associated solute‐binding protein of type I ABC transporter was originally identified in Chloroflexus aurantiacus, a phototrophic bacterium. Crystal structures in complex with CβGs revealed unique binding modes that are fundamentally distinct from that of the short β‐1,2‐glucooligosaccharides‐binding protein.
Kazuya Kato, Tatsuya Kaneko, Rintaro Hirayama, Nobukiyo Tanaka, Hiroyuki Nakai, Hidetaka Torigoe, Masahiro Nakajima   +6 more
wiley   +1 more source

16- DETECTION OF BRUCELLA SPECIES IN APPARENTLY HEALTHY COWS AND GOATS RAW MILK BY PCR [PDF]

Basrah Journal of Veterinary Research, 2018
Brucellosis an intracellular pathogen capable of infecting animals and humans. The aim of this study was to identify Brucella spp in apparently healthy cow and goat raw milk samples by a polymerase chain reaction (PCR)-based method.
Fawzia A. Abdullah, Enas Ismael Al- Jaboury   +1 more
doaj   +1 more source

Characterization of a phosphomonoesterase from Brucella abortus [PDF]

Infection and Immunity, 1990
Brucellae are facultative intracellular bacterial pathogens that reside primarily in cells of the reticuloendothelial system. The high-speed supernatant obtained after centrifuging a suspension of Brucella abortus that had been frozen-thawed and sonicated contained abundant phosphomonoesterase activity, determined by using 4-methylumbelliferylphosphate
A K, Saha, N K, Mukhopadhyay, J N, Dowling, T A, Ficht, L G, Adams, R H, Glew   +5 more
openaire   +2 more sources

Spatiotemporal Analysis of the Primary Factors and Risk Quantification of Human Brucellosis Prevention and Control in Zhejiang Province of China

Zoonoses and Public Health, EarlyView.
ABSTRACT Introduction Brucellosis is a major zoonotic disease that poses significant public health and socio‐economic threats. In China, human brucellosis incidence has continued to rise, including in highly developed regions such as Zhejiang Province, despite relatively low levels of livestock husbandry.
Juan Li, Wenjing Li, Lingyan Zhao, Wei Jiang, Yongzhong Song, Jimin Sun, Hongli Zhang, Huaiping Zhu   +7 more
wiley   +1 more source