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What's New? Vaginal high‐grade squamous intraepithelial lesions (HSILs), precursors of vaginal cancer that are related to human papillomavirus (HPV) infection, often are treated with laser vaporization. HSIL treated in this way, however, tends to recur.
Mari Kiviharju+7 more
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Printed electronics technology is known for its low cost, large area, ease of operation, and high speed, making it suitable for flexible electronic devices. Liquid metals, particularly gallium‐based alloys, are ideal for creating stretchable electronics due to their conductivity.
Chunxue Wan+5 more
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Applications of artificial intelligence‐driven microfluidics in medical laboratory science
The advancements in microfluidics and artificial intelligence are transforming the landscape of Medical Laboratory Science. This article systematically reviews the approaches and applications of AI‐driven microfluidics for analyzing cells, bacteria, nucleic acids, proteins, viruses, and cancer, while also addressing current challenges and proposing ...
Qingyang Jiang+9 more
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Using latent profile analysis, we investigated the co‐occurrence of adverse experiences in two UK cohorts (UKHLS and ALSPAC, total N > 15,000). We identified four profiles in both cohorts: low adversity, peer difficulties, sibling bullying, and poly‐adversity. 17%–22% of participants reported experiencing several social adversities.
Man Shiu Kwok+3 more
wiley +1 more source
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Purification of the human intestinal brush border membrane
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1973A method is described to obtain brush border,i.e. microvillus membranes, from human intestinal epithelial cells. It is based on the sequential use of CaCl2, differential centrifugation and Tris. The final preparation contains no DNA, no succinate dehydrogenase, traces only of (Na+K+)-ATPase, NADPH-cytochrome c reductase and β-glucuronidase and is ...
H. Preiser+5 more
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Isolation of Renal Brush Borders
Current Protocols in Cell Biology, 2006AbstractMethods are described to isolate intact brush borders and brush border membranes from renal cell homogenates. A rapid method yields sealed vesicles that reconstitute renal brush border transport. In one variation of this protocol, 10 to 20 mM CaCl2 or MgCl2 is added to aggregate non‐brush border structures for subsequent removal by ...
Timothy G. Hammond, D. James Morré
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Leakiness of brush-border vesicles
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1984From the water content of pelleted brush-border vesicles and from a comparison of the aqueous volume within the pellet that is available to [3H]inulin (58%), inulin [14C]carboxylic acid (34%, both approx. 5000 daltons), [3H]raffinose (97%, 540 daltons) and [3H]glucose (94%, 180 daltons) it is concluded that only 1 in 4 to 6 of the brush-border vesicles
Nigel Gains, Helmut Hauser
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Brush Border Particulates of Renal Tissue [PDF]
Particulates containing a large part of the alkaline phosphatase activity of renal tissue were separated from homogenates and from ribosomal preparations by zonal centrifugation. The particles had a high content of phospholipid and cholesterol that was not removed by treatment with 1 percent deoxycholate.
Reba K. Wright+5 more
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Isolation of Intestinal Brush‐Border Membranes
Current Protocols in Cell Biology, 2006AbstractThis unit provides protocols for isolating intestinal brush‐border membranes from rat, pig, and cow. These membranes can be used for immunoblotting or other analytical techniques. They will also spontaneously form closed vesicles which allow for flux assays to be performed using rapid filtration techniques. Overall the isolation procedures take
Debbie O'Neill, Chris I. Cheeseman
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