Results 201 to 210 of about 2,471,677 (248)

Development of a Direct Cell-to-PCR Lysis Buffer Using Optimized Non-Ionic Detergents for RNA-Extraction-Free RT-qPCR. [PDF]

Methods Protoc
Zhra M, Alarawi RA, Mohamed SA, Daoud AN, Fakhoury H, Aljada A.   +5 more
europepmc   +1 more source

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

Hydrolysis-based degradation of organophosphorus nerve agents and simulants: water/base-dependent reactivity, catalytic materials and life-cycle considerations. [PDF]

RSC Adv
Kwak JW, Lee JB, Park BC, Cha JH, Jeon SG, Kim GH, Park YD, Oh K, Park JI.   +8 more
europepmc   +1 more source

Effect of terahertz irradiation on DNA damage repair in living cells

FEBS Open Bio, EarlyView.
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno, Shota Yamazaki, Hiromichi Hoshina, Masahiko Harata   +3 more
wiley   +1 more source

Proton Transport as a Missing Design Variable in Immobilized Enzyme Catalysis. [PDF]

Chembiochem
Wiśniewski M.
europepmc   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Correction to "Insights into the regulatory roles of LIKE-HETEROCHROMATIN PROTEIN 1 and its targeting to different nuclear compartments modulated by NLS and the conserved domains in the moss Physcomitrium patens". [PDF]

Plant J
europepmc   +1 more source

Comprehensive immune profiling of human peripheral blood mononuclear cells using two complementary spectral flow cytometry panels encompassing 60 unique markers. [PDF]

Front Immunol
Rietdijk MH, Kuhnen LC, van den Braber M, Paul AGA, Pascutti MF, García-Vallejo JJ.   +5 more
europepmc   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

Scalable Production of Transfection-Grade Plasmid DNA by Liquid-Liquid Extraction Without Chromatography: Application to CAR-T Vector Packaging. [PDF]

Bioengineering (Basel)
Nigmatulla G, Kydyrbayeva A, Kulatay T, Zauatbayeva G, Ingirbay B, Sagyndykova L, Keyer V, Zharlyganova D, Zhumabekova M, Shustov AV.   +9 more
europepmc   +1 more source