Results 181 to 190 of about 453,832 (299)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Residential greenness and atopic dermatitis in Japanese children: Findings from the TMM BirThree cohort study. [PDF]

Pediatr Allergy Immunol
Uematsu A, Ohseto H, Orui M, Xian Z, Takahashi Y, Chen G, Murakami K, Ishikuro M, Noda A, Shinoda G, Obara T, Nakaya T, Kuriyama S.   +12 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Using deep learning to explore the impacts of street-view green space on school myopia prevalence: a multicenter, cross-sectional study. [PDF]

Sci Rep
Hua D, Yang T, Cui Q, Zhang J, Luo G, Cheng A, Su W, Ming M, Zhou F, Zhang R, Yuan Z, Chen C, Xu Y, Chen Z.   +13 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Active Plasmonic Surfaces via Electrically Driven Actuation of DNA-Tethered Nanoparticles. [PDF]

ACS Nano
Al Hussain MMA, Kipnis A, Lumppio A, Witwiyaruj N, Manuguri S, Pham XH, Bléteau P, Fauconnier M, Asgari M, Asadchy V, Kuzyk A, Tapio K.   +11 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

sc-rDSeq: Droplet-based single-cell full-length total RNA-seq method. [PDF]

Biol Methods Protoc
Sun X, Ram O.
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Protocol for total RNA sequencing analysis of extracellular RNA from biofluids. [PDF]

STAR Protoc
Decock A, Verniers K, Decruyenaere P, Morlion A, Verwilt J.   +4 more
europepmc   +1 more source