Results 91 to 100 of about 4,114 (215)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Synthesis of Multi-Criteria Decision-Making Applications in Facilities Management and Building Maintenance: Trends, Methods, and Future Research Directions

Buildings
Building maintenance decisions are complex and often influenced by various factors. Multi-criteria decision-making (MCDM) methods have been widely applied to address this complexity, yet guidance on selecting the most appropriate method for specific ...
Mahdi Anbari Moghadam, Deniz Besiktepe
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Enhancing Cost Prediction and Estimation Techniques for Sustainable Building Maintenance and Future Development

Encyclopedia
Building maintenance is crucial, yet predicting financial resources for it remains challenging, particularly during the design and construction phases. This research aims to analyze and synthesize existing studies on maintenance cost estimation, with a ...
Dino Obradović, Hana Begić Juričić, Hrvoje Krstić   +2 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Advantages of using insulation with lower thermal conductivity versus WT 2021 – comparison of the costs of materials, buying and operating a heating device, and a carbon footprint generated

Budownictwo i Architektura
This article presents a comparative analysis of the use of standard insulation material compliant with the current Technical Conditions WT 2021, compared to insulation material with improved thermal conductivity parameters on building partitions.
Patrycja Zawiska
doaj   +1 more source

Design Failure Affecting Maintenance Management on Public Higher Education Institution in Malaysia

MATEC Web of Conferences, 2016
The issue of government building defect is keeping on reporting in the media and arising since 2007. These issues of building defects, maintenance and management have existing and it is deliberated up to the parliament level. The government keep continue
Agus Salim Nuzaihan Aras, Salleh Naziah Muhamad, Zahari Nurul Fadzila   +2 more
doaj   +1 more source

AscF in the mycobacterial CIII–CIV supercomplex lacks metal and nucleotide binding but links malate oxidation to respiration

FEBS Letters, EarlyView.
The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile, Riccardo Diamanti, Daniel Lundin, Soni Sharma, Robin Lissner, Coenraad P. Kuil, Martin Högbom, Dirk Bald, Dan Sjöstrand   +8 more
wiley   +1 more source

Design of Structural Damage Identification Applications Web-Based

MATEC Web of Conferences, 2018
The current building construction and maintenance Note well, certainly will be utilized as desired. If a building is experiencing interference, then all activities will be obstructed so that the building requires maintenance activities.
Anisya Anisya, Nugroho Fajar
doaj   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source