Results 181 to 190 of about 5,883 (195)

Development of sheep kidney cells with increased resistance to different subgenotypes of BVDV-1 by RNA interference

Journal of Virological Methods, 2015
Bovine viral diarrhea virus (BVDV) should be a ubiquitous viral pathogen to the cattle and sheep industry. This pathogen is responsible for severe economic losses. We previously showed that plasmid-mediated dual short hairpin RNA (shRNA) efficiently inhibit BVDV replication in bovine kidney epithelial (MDBK) cells.
Wei, Ni, Jun, Qiao, Qiman, Ma, Jiangde, Wang, Dawei, Wang, Xinxia, Zhao, Yang, Cao, Qifeng, Li, Shengwei, Hu, Chuangfu, Chen   +9 more
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Pathogenetic differences after experimental infection of calves with Korean non-cytopathic BVDV-1 and BVDV-2 isolates

Veterinary Immunology and Immunopathology, 2013
The purpose of this study was to compare clinical and virological differences between non-cytopathic (ncp) bovine viral diarrhea virus (BVDV)-1 and ncp BVDV-2 isolated from Korean field cases. Each five naïve calves were experimentally infected with Korean ncp BVDV-1 or BVDV-2 isolates.
Giyong, Seong, Jae-Ku, Oem, Kyoung-Seong, Choi   +2 more
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Investigation of a dual fetal infection model with bovine viral diarrhoea viruses (BVDV)-1 and BVDV-2

Archives of Virology, 2011
Two studies were performed in pregnant heifers to determine whether inoculation with two bovine viral diarrhoea viruses (BVDV), one BVDV-1 and one BVDV-2, inoculated separately into either nostril, results in fetal infection with both viruses.
B, Makoschey, M G J, Janssen
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Cytopathic BVDV-1 strain induces immune marker production in bovine cells through the NF-κB signaling pathway

Molecular Immunology, 2015
The bovine viral diarrhea virus (BVDV-1) is a pathogen responsible for high economic losses in the cattle industry worldwide. This virus has the capacity to modulate the immune system of several higher vertebrates, but there is little information available on the cell infection mechanism.
Fernanda, Fredericksen, Gonzalo, Carrasco, Melina, Villalba, Víctor H, Olavarría   +3 more
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Transcriptomic analysis of responses to cytopathic bovine viral diarrhea virus-1 (BVDV-1) infection in MDBK cells

Molecular Immunology, 2016
The bovine viral diarrhea virus (BVDV) is responsible for significant economic losses in the dairy and cattle industry; however, little is known about the protective and pathological responses of hosts to infection. The present study determined the principal molecular markers implicated in viral infection through meta-transcriptomic analysis using MDBK
Melina, Villalba, Fernanda, Fredericksen, Carola, Otth, Víctor, Olavarría   +3 more
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A newly developed BVDV-1 RT-qPCR Taqman assay based on Italian isolates: evaluation as a diagnostic tool

Folia Microbiologica, 2017
A single-step TaqMan® RT-qPCR was developed for the detection of bovine viral diarrhea virus type 1 (BVDV-1), an important pathogen of cattle worldwide. The assay was based on conserved 5'UTR sequences of Italian BVDV-1 isolates. In order to establish a diagnostic protocol which simplifies sample collection and processing, the assay was tested on a ...
Zoccola, Roberto, MAZZEI, MAURIZIO, Carrozza, Maria Luisa, Ricci, Emanuele, FORZAN, MARIO, PIZZURRO, FEDERICA, Giammarioli, Monica, BANDECCHI, PATRIZIA, TOLARI, FRANCESCO   +8 more
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Genetic heterogeneity of bovine viral diarrhoea virus (BVDV) isolates from Turkey: Identification of a new subgroup in BVDV-1

Veterinary Microbiology, 2008
Genetic heterogeneity of Turkish ruminant pestiviruses was investigated by phylogenetic analysis of complete N(pro) encoding nucleotide sequences. A total of 30 virus isolates obtained from 15 provinces around the country between 1997 and 2005 were included in the phylogenetic analysis. Virus isolates mostly originated from cattle with one isolate from
YEŞİLBAĞ, KADİR, Yllmaz, ZEKİ, Koenig, Matthias, Thiel, Heinz-Juergen, Rosales, Sibilina Cedillo, BURGU, İBRAHİM, ÖZKUL, AYKUT, ALKAN, FERAY, Bank-Wolf, Barbara, Foerster, Christine   +9 more
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Development of a RT-PCR ELISA for simultaneous detection of BVDV-1, BVDV-2 and BDV in ruminants and its evaluation on clinical samples

Journal of Virological Methods, 2015
The aim of this study was to develop a reverse transcription polymerase chain reaction ELISA (RT-PCR ELISA) for detection of ruminant pestiviruses and to evaluate its diagnostic performance on clinical samples obtained from cattle, sheep and goats.
Pooja, Dubey, N, Mishra, K, Rajukumar, S P, Behera, S, Kalaiyarasu, R K, Nema, A, Prakash   +6 more
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Effects of oocytes exposure to bovine diarrhea viruses BVDV-1, BVDV-2 and Hobi-like virus on in vitro-produced bovine embryo development and viral infection

Theriogenology, 2017
As production of in vitro (IVP) bovine embryos steadily increases, the sanitary risk associated with IVP embryos remains a concern. One of the greatest concerns is how BVDV may be transmitted through IVP embryos. The objective of this study was to evaluate the effects caused by BVDV-1, BVDV-2 and Hobi-like virus exposure during in vitro maturation on ...
V, da Silva Cardoso Pinto, M F, Alves, M, de Souza Nunes Martins, A C, Basso, J H, Tannura, J H F, Pontes, M Santos, Lima, T, Garcia da Silva, L H, Okuda, E, Stefano, A H C N, Romaldini, D R, Arnold, E M, Pituco   +12 more
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The effects of reference genes in qRT-PCR assays for determining the immune response of bovine cells (MDBK) infected with the Bovine Viral Diarrhea Virus 1 (BVDV-1)

Gene, 2015
The bovine viral diarrhea virus (BVDV) causes significant economic losses to the dairy industry worldwide, and understanding its infection mechanisms would be extremely useful in designing new and efficient treatments. Due to the limited number of specific antibodies against bovine proteins, differential gene expression analyses are vital for ...
Fernanda, Fredericksen, Fredy, Delgado, Cristian, Cabrera, Alejandro, Yáñez, Carrasco, Gonzalo, Melina, Villalba, Víctor H, Olavarría   +6 more
openaire   +2 more sources