Novel BVDV-2 mutants as new candidates for modified-live vaccines
Protection against Bovine viral diarrhea virus (BVDV) type 2 infection of commercially available vaccines is often limited due to marked genetic and antigenic differences between BVDV types 1 (BVDV-1) and 2 (BVDV-2). Therefore, the immunogenicity of selected BVDV-1 and BVDV-2 mutants derived from infectious full-length cDNA clones and their use as ...
Zemke, Johanna +4 more
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Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control [PDF]
<p>The current study reports on a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories.
Beer, Martin +18 more
core +4 more sources
Global Transcriptomic Profiling of Bovine Endometrial Immune Response In Vitro. II. Effect of Bovine Viral Diarrhea Virus on the Endometrial Response to Lipopolysaccharide [PDF]
Infection with noncytopathic bovine viral diarrhea virus (ncpBVDV) is associated with uterine disease and infertility. This study investigated the influence of ncpBVDV on immune functions of the bovine endometrium by testing the response to bacterial ...
Abudureyimu, A +6 more
core +2 more sources
Additional file 6: Figure S1. The expression of IL17A was detected by western blot in PBLCs at 12 h, 24 h and 48 h after GS2018 infection, respectively. The above showing original blots of the targets protein. The band of IL17A (a, b) and β-actin (c, d) were cropped horizontally from the same membrane before hybridized with the antibody and then ...
Li, Yanping +8 more
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Comparação dos testes de virusneutralização contra os genótipos 1 e 2 do vírus da diarreia viral bovina (BVDV-1 e BVDV-2) em bovinos de rebanhos naturalmente infectados Comparison of virusneutralization tests against genotypes 1 and 2 of the bovine viral diarrhoea virus (BVDV-1 and BVDV-2) in cattle of naturally infected herds [PDF]
Os resultados dos testes de virusneutralização (VN) contra os genótipos do vírus da diarreia viral bovina (BVDV-1 e BVDV-2), bem como os respectivos títulos de anticorpos, foram comparados em 1.925 amostras de soro sanguíneo obtidas de rebanhos bovinos naturalmente infectados e não vacinados contra o BVDV, provenientes dos Estados de São Paulo e Minas ...
Fabio Carvalho Dias +5 more
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Pathogenic mechanisms implicated in the intravascular coagulation in the lungs of BVDV-infected calves challenged with BHV-1 [PDF]
Resistance to respiratory disease in cattle requires host defense mechanisms that protect against pathogens which have evolved sophisticated strategies to evade them, including an altered function of pulmonary macrophages (MΦs) or the induction of ...
Garfia, Bartolomé +6 more
core +1 more source
Additional file 7: Figure S2. Overexpression of IL17A inhibited the replication of GS2018. The Expression of IL17A and BVDV-2 E2 were detected by western blot in PBLCs at 24 h, 48 h and 72 h after GS2018 infection, respectively. The band of IL17A (a), BVDV-2 E2 (b, c) and GAPDH (d) protein were cropped horizontally from the same membrane before ...
Li, Yanping +8 more
openaire +1 more source
International proficiency trial for bovine viral diarrhea virus (BVDV) antibody detection: limitations of milk serology [PDF]
Background Control programs were implemented in several countries against bovine viral diarrhea (BVD), one of the most significant cattle diseases worldwide. Most of the programs rely on serological diagnostics in any phase of the program.
Beer, Martin, Wernike, Kerstin
core +1 more source
Additional file 8: Table S7. Primers used for qRT-PCR.
Li, Yanping +8 more
openaire +1 more source
An outbreak of abortions, stillbirths and malformations in a Spanish sheep flock associated with a bovine viral diarrhoea virus 2-contaminated orf vaccine [PDF]
Bovine viral diarrhoea virus (BVDV) is a pestivirus that affects both cattle and sheep, causing an array of clinical signs, which include abortions and malformations in the offspring. Manufacturing of modified live virus (MLV) vaccines often includes the
Akerman, A. +8 more
core +1 more source

