Results 81 to 90 of about 11,319 (119)

登革病毒NS1蛋白的原核表达及其在登革热快速诊断中的应用

Zhongshan Daxue xuebao. Yixue kexue ban, 2012
【目的】 重组表达1型登革病毒(DENV-1)和3型登革病毒(DENV-3)NS1蛋白,制备多克隆抗体,建立特异、敏感的登革病毒NS1抗原ELISA检测法。【方法】 RT-PCR扩增DENV-1和DENV-3 NS1全长基因,经T-A克隆后,将目的基因分别与质粒PET30a(+)连接构建重组质粒,转化大肠杆菌BL21感受态细胞,阳性克隆经IPTG诱导表达,并对目的蛋白进行纯化，用纯化的NS1蛋白免疫BALB/c小鼠,制备鼠抗NS1多克隆抗体 ...
doaj  

[Gambogic acid protects against lipopolysaccharide-induced acute lung injury via the MAPK signaling pathway]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Wang L, Wen HW, Yu LS, Deng DD, Yu XD, Liu J.   +5 more
europepmc   +1 more source

SCUBE1、Occludin在急性缺血性脑卒患者中的表达及与促动脉硬化指数的关系

Zhongguo shiyan zhenduanxue
目的分析信号肽Cub-Egf结构域蛋白1（Signal peptide Cub Elf domain protein 1，SCUBE1）、闭合蛋白（Occludin）在急性缺血性脑卒中患者中的表达及与促动脉硬化指数（Arteriosclerosis index，AI）的关系。方法选取齐齐哈尔市中医院2021年5月至2023年5月收治的72例急性缺血性脑卒中患者为研究组，另外选取50例健康体检者作为对照组，检查两组血清SCUBE1、Occludin、超敏C反应蛋白、白细胞计数、D ...
马春艳
doaj  

[Growth plate regulation and short stature: mechanistic insights and therapeutic advances]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Mo GS, Ma YT, Gui BH.
europepmc   +1 more source

[<i>Xiaoyao San</i> alleviates abnormal α‑syn aggregation and neuronal injury in a <i>Caenorhabditis</i> elegans model of Parkinson disease <i>via</i> the SKN-1 antioxidative pathway]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Ye L, Liao Y, Liu Q, Zhang J, Gao L, Dong Z.   +5 more
europepmc   +1 more source

[Research advances in immune and metabolic dysregulation in the diagnosis and treatment of acute-on-chronic liver failure]. [PDF]

Zhonghua Gan Zang Bing Za Zhi
Wang SR, Jia H, Zhang X, Yang H, Li JW, Liu RY, Shen M, Han ZZ, Wang XY, Yuan W, Liu M, Zhou L, Ran Y.   +12 more
europepmc   +1 more source

[Anti-inflammatory Mechanisms of Human Resistin Through Activation of Peroxisome Proliferator-Activated Receptor γ]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Shi B, Han B, Liu Y, Liu R.
europepmc   +1 more source

[Activation of the CaMKKβ/AMPK/HIF-1α signaling pathway promotes angiogenesis in endometriosis by regulating mitophagy]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Chen Y, Yue B, Wu Q, Mao H, Ji X, Chu C, Chen L, Zhang Y, Li B, Zhang X, Feng P.   +10 more
europepmc   +1 more source

[The E3 ubiquitin ligase KLHL8 degrades HBx protein via the ubiquitin-proteasome pathway and inhibits HBV cccDNA transcription]. [PDF]

Zhonghua Gan Zang Bing Za Zhi
Liu ZH, He XM, Zhao B, Li JQ, Xu KX, Yu HB, Chen J.   +6 more
europepmc   +1 more source

[CHOP promotes hepatocyte pyroptosis by downregulating endoplasmic reticulophagy and enhancing endoplasmic reticulum-mitochondria interaction]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Guo X, Wu J, Han B, Yang T, Yin H, Xiong J, Xie R.   +6 more
europepmc   +1 more source