Results 131 to 140 of about 3,165,692 (263)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Editorial: Remembering John C. Light. I. Editorial contributions [PDF]

The Journal of Chemical Physics, 2016
openaire   +2 more sources

Assessing ultraviolet-C light-emitting diode disinfection of disposable video laryngoscope blades: a sustainable approach through an integrated microbiological, environmental, economic, and regulatory evaluation. [PDF]

BJA Open
Siwe H, Delbeecke B, Cools P, Meuleman P, Verdonck P, Kalmar AF.   +5 more
europepmc   +1 more source

Tolerance of clinical vancomycin-resistant Enterococcus faecium isolates against UV-C light from a mobile source. [PDF]

Antimicrob Resist Infect Control, 2023
Knobling B, Franke G, Belmar Campos C, Büttner H, Christner M, Klupp EM, Maurer PM, Knobloch JK.   +7 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Antimicrobial Activity of Ultraviolet C Light as a Potential Novel Treatment for Moraxella bovis Infection-An In Vitro Study. [PDF]

Vet Ophthalmol
Turicea B, Sahoo DK, Allbaugh RA, Stinman CC, Kubai MA.   +4 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Bactericidal Efficacy of Ultraviolet-C Light on Virtual Reality Devices: In Vitro Assessment of Bacterial Killing. [PDF]

JMIR Form Res
Roberts SC, Wright J, Mustafa M, Feinn RS, Marks A, Hieftje K, Huang PH, Martinello RA, Murray TS, HIPSTER Team.   +9 more
europepmc   +1 more source

Solid-State Far-Ultraviolet C Light Sources for the Disinfection of Pathogenic Microorganisms Using Graphene Nanostructure Field Emitters. [PDF]

Glob Chall, 2023
Neo Y, Hashimoto G, Koike R, Ohhara T, Matsumoto T.   +4 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source