Results 111 to 120 of about 45,573,897 (286)

Multiscale mechanistic insights into coal gangue for enhancing engineered geopolymer composites (EGCs): nano, micro and macro property correlations

open access: yesMaterials & Design
In this paper, coal gangue (CG) was used to prepare Engineering Polymer Composites (EGCs). The nano, micro, and macro properties were elucidated through macro-mechanical testing and microstructural characterization.
Li Wang   +3 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Study on the performance of basalt fiber geopolymer concrete by freeze-thaw cycle coupled with sulfate erosion

open access: yesAIP Advances
In this research, double-blended materials such as fly ash and slag are used as binders, and basalt fibers (BF) are mixed in varying volume fractions (0.2%, 0.4%, 0.6%, 0.8%, and 1.0%) relative to the volume of concrete. Besides, sodium silicate (Na2SiO3)
Yadong Bian   +4 more
doaj   +1 more source

Study on the advancement process of alkali loss and deterioration of concrete under MgSO4 erosion

open access: yesJournal of Materials Research and Technology
To clarify the damage mechanism of the layer erosion argillization of concrete eroded by sulfate attack, this study investigates the compressive strength decay characteristics of eroded concrete.
Zhongzhe Zhang   +5 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

open access: yesFEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose   +9 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

open access: yesFEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard   +12 more
wiley   +1 more source

Micromechanical characterization of γ-irradiated cement paste exposed to different relative humidity conditions

open access: yesActa Polytechnica CTU Proceedings
In this study, well-hydrated cement paste samples made from Portland cement CEM I-42.5R were gamma irradiated with a 200 TBq 60Co source, resulting in a total absorbed dose of 13.82MGy. During irradiation, the samples were stored under specific relative
Jiří Němeček   +3 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

open access: yesFEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya   +2 more
wiley   +1 more source

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