Results 281 to 290 of about 523,466 (372)

Label‐Free and Immobilization‐Free Protein‐Binding Assays by Ultraviolet Transient Absorption Microscopy

open access: yesAdvanced Science, EarlyView.
A femtosecond UV pump–probe microscope resolves binding‐induced changes in protein excited‐state dynamics and converts these intrinsic contrasts into an equilibrium affinity readout. By probing tryptophan responses directly in solution, the approach enables quantitative determination of dissociation constants without labeling or immobilization ...
Jianghao Shen   +5 more
wiley   +1 more source

Clickable Microgel Inks Enable Spatioselective, Multi‐Stimuli Programmable Assembly of Materials

open access: yesAdvanced Science, EarlyView.
Clickable microgel inks enable direct ink writing of hydrogel architectures with intrinsic spatioselective and programmable multi‐responsiveness. By combining pH‐responsive and temperature‐responsive microgel building blocks through Diels‐Alder interparticle crosslinking, the assemblies exhibit controllable swelling and shape changes.
Junho Moon   +3 more
wiley   +1 more source

Development and Structural Characterization of UTE‐156, a Covalent Inhibitor of the VCP/p97 AAA+ ATPase

open access: yesAdvanced Science, EarlyView.
The AAA+ ATPase Valosin‐containing protein (VCP/p97) regulates protein homeostasis by unfolding ubiquitinated substrates. Here, we describe UTE‐156, a novel irreversible covalent inhibitor that modifies Cys522 in the D2 ATPase motor domain. Although its pharmacochemical limitations preclude immediate therapeutic use, UTE‐156 serves as a valuable ...
Daniela Tamayo‐Jaramillo   +8 more
wiley   +1 more source

Oil‐Coated Nanoplastics Induce Rapid Membrane Disruption and Severe Intestinal Injury

open access: yesAdvanced Science, EarlyView.
ABSTRACT Plastic food containers used in food delivery may pose underrecognized health risks by releasing micro‐ and nanoplastics (MNPs), especially when in contact with oil‐rich foods. Here, we show that cooking oil dramatically increased MNP release from polypropylene (PP) and polyethylene (PE)‐coated containers within 3 min of microwave heating ...
Ruwen Xie   +17 more
wiley   +1 more source

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