Results 51 to 60 of about 29,275 (154)

Dominant and novel clades of Candidatus Accumulibacter phosphatis in 18 globally distributed full-scale wastewater treatment plants. [PDF]

open access: yesSci Rep, 2015
Here we employed quantitative real-time PCR (qPCR) assays for polyphosphate kinase 1 (ppk1) and 16S rRNA genes to assess relative abundances of dominant clades of Candidatus Accumulibacter phosphatis (referred to Accumulibacter) in 18 globally ...
Mao Y, Graham DW, Tamaki H, Zhang T.
europepmc   +2 more sources

ATP‐Independent and Cell‐Free Biosynthesis of β‐Hydroxy Acids Using Vinyl Esters as Smart Substrates

open access: yesAngewandte Chemie, Volume 135, Issue 13, March 20, 2023., 2023
A 4‐enzyme cell‐free system transforms vinyl esters into β‐hydroxy acids in the absence of ATP and ancillary electron donors through concurrent abiotic thiolysis, non‐decarboxylating Claisen condensation, ketone reduction and hydrolysis steps. The same substrate provides the energy to activate the acyl group and the redox power to reduce the condensed ...
Alejandro H. Orrego   +7 more
wiley   +2 more sources

Metabolic Differentiation of Co-occurring Accumulibacter Clades Revealed through Genome-Resolved Metatranscriptomics

open access: yesmSystems, 2021
Natural microbial communities consist of closely related taxa that may exhibit phenotypic differences and inhabit distinct niches. However, connecting genetic diversity to ecological properties remains a challenge in microbial ecology due to the lack of ...
Elizabeth A. McDaniel   +8 more
doaj   +1 more source

Metaproteomic Profiling of the Secretome of a Granule-forming Ca. Accumulibacter Enrichment. [PDF]

open access: yesProteomics
ABSTRACT Extracellular proteins are supposed to play crucial roles in the formation and structure of biofilms and aggregates. However, often little is known about these proteins, in particular for microbial communities. Here, we use two advanced metaproteomic approaches to study the extracellular proteome in a granular Candidatus Accumulibacter ...
van Olst B   +7 more
europepmc   +2 more sources

Oligotyping and metagenomics reveal distinct Candidatus Accumulibacter communities in side-stream versus conventional full-scale enhanced biological phosphorus removal (EBPR) systems.

open access: yesWater Research, 2021
Candidatus Accumulibacter phosphatis (CAP) and its clade-level micro-diversity has been associated with and implicated in functional differences in phosphorus removal performance in enhanced biological phosphorus removal (EBPR) systems. Side-stream EBPR (
Varun N. Srinivasan   +10 more
semanticscholar   +1 more source

Bacterial adaptation to cold: Conservation of a short J‐domain co‐chaperone and its protein partners in environmental proteobacteria

open access: yesEnvironmental Microbiology, Volume 25, Issue 11, Page 2447-2464, November 2023., 2023
AtcJ is a short J‐domain protein essential for cold growth in S. oneidensis, conserved in environmental β‐ and γ‐proteobacteria, and encoded in an operon along with its protein partners AtcA, AtcB, and AtcC. The AtcJ superfamily contains a conserved PX7W motif whose Trp ensures the high affinity of the AtcJ–AtcC interaction, which is crucial for growth
Lana Weber   +10 more
wiley   +1 more source

Metaproteomics provides functional insight into activated sludge wastewater treatment. [PDF]

open access: yesPLoS ONE, 2008
Through identification of highly expressed proteins from a mixed culture activated sludge system this study provides functional evidence of microbial transformations important for enhanced biological phosphorus removal (EBPR).A laboratory-scale ...
Paul Wilmes   +2 more
doaj   +1 more source

Wastewater treatment potential, light penetration profile and biomass settling performance of a photo‐sequencing batch reactor

open access: yesJournal of Chemical Technology &Biotechnology, Volume 98, Issue 2, Page 346-356, February 2023., 2023
Abstract BACKGROUND The performance of microalgal and bacterial systems devoted to wastewater treatment might be limited by the poor settling of microalgal biomass and the inhibition of nitrifying bacteria under high irradiance conditions. This work implemented a photo‐sequencing batch bioreactor (PSBR) treating medium‐ and low‐strength wastewaters ...
Alfonso García   +7 more
wiley   +1 more source

Unmasking photogranulation in decreasing glacial albedo and net autotrophic wastewater treatment

open access: yesEnvironmental Microbiology, Volume 23, Issue 11, Page 6391-6404, November 2021., 2021
Summary In both natural and built environments, microbes on occasions manifest in spherical aggregates instead of substratum‐affixed biofilms. These microbial aggregates are conventionally referred to as granules. Cryoconites are mineral rich granules that appear on glacier surfaces and are linked with expanding surface darkening, thus decreasing ...
Chul Park, Nozomu Takeuchi
wiley   +1 more source

DIAMOND+MEGAN: Fast and Easy Taxonomic and Functional Analysis of Short and Long Microbiome Sequences

open access: yesCurrent Protocols, Volume 1, Issue 3, March 2021., 2021
Abstract One main approach to computational analysis of microbiome sequences is to first align against a reference database of annotated protein sequences (NCBI‐nr) and then perform taxonomic and functional binning of the sequences based on the resulting alignments.
Caner Bağcı   +2 more
wiley   +1 more source

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