Reaction of pyranose dehydrogenase from Agaricus meleagris with its carbohydrate substrates
Monomeric Agaricus meleagris pyranose dehydrogenase (AmPDH) belongs to the glucose–methanol–choline family of oxidoreductases. An FAD cofactor is covalently tethered to His103 of the enzyme. AmPDH can double oxidize various mono‐ and oligosaccharides at different positions (C1 to C4). To study the structure/function relationship of selected active‐site
Michael M.H. Graf +11 more
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Crystal structure of quinone‐dependent alcohol dehydrogenase from
AbstractThe quinone‐dependent alcohol dehydrogenase (PQQ‐ADH, E.C. 1.1.5.2) from the Gram‐negative bacterium Pseudogluconobacter saccharoketogenes IFO 14464 oxidizes primary alcohols (e.g. ethanol, butanol), secondary alcohols (monosaccharides), as well as aldehydes, polysaccharides, and cyclodextrins.
Rozeboom, Henriette J. +5 more
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Modulation of plant growth in vivo and identification of kinase substrates using an analog-sensitive variant of CYCLIN-DEPENDENT KINASE A;1 [PDF]
BACKGROUND: Modulation of protein activity by phosphorylation through kinases and subsequent de-phosphorylation by phosphatases is one of the most prominent cellular control mechanisms.
Dissmeyer, N. +6 more
core +2 more sources
AcDCXR Is a Cowpea Aphid Effector With Putative Roles in Altering Host Immunity and Physiology
Cowpea, Vigna unguiculata, is a crop that is essential to semiarid areas of the world like Sub-Sahara Africa. Cowpea is highly susceptible to cowpea aphid, Aphis craccivora, infestation that can lead to major yield losses. Aphids feed on their host plant
Jacob R. MacWilliams +6 more
doaj +1 more source
Quantitative RNA-seq Analysis Unveils Osmotic and Thermal Adaptation Mechanisms Relevant for Ectoine Production in Chromohalobacter salexigens [PDF]
Quantitative RNA sequencing (RNA-seq) and the complementary phenotypic assays were implemented to investigate the transcriptional responses of Chromohalobacter salexigens to osmotic and heat stress.
Carmen Vargas +6 more
core +2 more sources
Adaptation of pine wood nematode Bursaphelenchus xylophilus to β-pinene stress
Background The pine wood nematode (PWN; Bursaphelenchus xylophilus) is the most damaging biological pest in pine forest ecosystems in China. However, the pathogenic mechanism remains unclear.
Yongxia Li +5 more
doaj +1 more source
Global analysis of mannitol 2-dehydrogenase in lactobacillus reuteri crl 1101 during mannitol production through enzymatic, genetic and proteomic approaches [PDF]
Several plants, fungi, algae, and certain bacteria produce mannitol, a polyol derived from fructose. Mannitol has multiple industrial applications in the food, pharmaceutical, and medical industries, being mainly used as a non-metabolizable sweetener in ...
Bleckwedel, Juliana +6 more
core +3 more sources
Transcriptomic and proteomic analyses of Desulfovibrio vulgaris biofilms: carbon and energy flow contribute to the distinct biofilm growth state. [PDF]
BackgroundDesulfovibrio vulgaris Hildenborough is a sulfate-reducing bacterium (SRB) that is intensively studied in the context of metal corrosion and heavy-metal bioremediation, and SRB populations are commonly observed in pipe and subsurface ...
Arkin, Adam P +8 more
core +3 more sources
The Carbohydrate Intermediates of Erythrocytes Deficient in Glucose-6-Phosphate Dehydrogenase [PDF]
Abstract The carbohydrate intermediates of erythrocytes were studied before and after incubation for 6 hours at 37 C. A comparison was made between a case of NSHA with G-6-PD deficiency, his mother, a Caucasian and a Negro with G-6-PD deficiency (so-called primaquine sensitivity), and a normal case.
openaire +2 more sources
Pyranose Dehydrogenase from Agaricus campestris and Agaricus xanthoderma: Characterization and Applications in Carbohydrate Conversions [PDF]
Pyranose dehydrogenase (PDH) is a flavin-dependent sugar oxidoreductase that is limited to a rather small group of litter-degrading basidiomycetes. The enzyme is unable to utilize oxygen as an electron acceptor, using substituted benzoquinones and (organo) metal ions instead.
Clemens K. Peterbauer +3 more
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